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苯丙氨酸解氨酶 2.1 有助于大豆对大豆疫霉感染的反应。

Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection.

机构信息

Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, Heilongjiang, China.

Heilongjiang Academy of Land Reclamation Sciences, Harbin, Heilongjiang, China.

出版信息

Sci Rep. 2017 Aug 3;7(1):7242. doi: 10.1038/s41598-017-07832-2.

DOI:10.1038/s41598-017-07832-2
PMID:28775360
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5543151/
Abstract

Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecular mechanism of PAL in soybean in response to P. sojae is largely unclear. Here, we characterize a novel member of the soybean PAL gene family, GmPAL2.1, which is significantly induced by P. sojae. Overexpression and RNA interference analysis demonstrates that GmPAL2.1 enhances resistance to P. sojae in transgenic soybean plants. In addition, the PAL activity in GmPAL2.1-OX transgenic soybean is significantly higher than that of non-transgenic plants after infection with P. sojae, while that in GmPAL2.1-RNAi soybean plants is lower. Further analyses show that the daidzein, genistein and salicylic acid (SA) levels and the relative content of glyceollins are markedly increased in GmPAL2.1-OX transgenic soybean. Taken together, these results suggest the important role of GmPAL2.1 functioning as a positive regulator in the soybean response to P. sojae infection, possibly by enhancing the content of glyceollins, daidzein, genistein and SA.

摘要

大豆疫霉根腐和茎腐病（Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by Phytophthora sojae）是一种全球性的破坏性疾病。苯丙氨酸解氨酶（Phenylalanine ammonia-lyase，PAL）是与植物对生物和非生物胁迫反应相关的研究最广泛的酶之一。然而，大豆中 PAL 对大豆疫霉的分子机制在很大程度上尚不清楚。在这里，我们描述了大豆 PAL 基因家族的一个新成员 GmPAL2.1，它被大豆疫霉显著诱导。过表达和 RNA 干扰分析表明，GmPAL2.1 增强了转基因大豆植株对大豆疫霉的抗性。此外，在感染大豆疫霉后，GmPAL2.1-OX 转基因大豆中的 PAL 活性明显高于非转基因植物，而 GmPAL2.1-RNAi 大豆植物中的 PAL 活性较低。进一步的分析表明，GmPAL2.1-OX 转基因大豆中的大豆苷元、染料木黄酮和水杨酸（salicylic acid，SA）水平以及大豆异黄酮的相对含量显著增加。综上所述，这些结果表明 GmPAL2.1 作为大豆对大豆疫霉感染反应的正调控因子发挥重要作用，可能通过增强大豆苷元、染料木黄酮、SA 和大豆异黄酮的含量来实现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/7d9dc8bc6c5d/41598_2017_7832_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/14c6eae6b187/41598_2017_7832_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/7c0ea861b856/41598_2017_7832_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/894411dda59c/41598_2017_7832_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/f2a20191884c/41598_2017_7832_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/71319d1422c5/41598_2017_7832_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/5a20b044fbc8/41598_2017_7832_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/b959cc9a4987/41598_2017_7832_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/7d9dc8bc6c5d/41598_2017_7832_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/14c6eae6b187/41598_2017_7832_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/7c0ea861b856/41598_2017_7832_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/894411dda59c/41598_2017_7832_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/f2a20191884c/41598_2017_7832_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/71319d1422c5/41598_2017_7832_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/5a20b044fbc8/41598_2017_7832_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/b959cc9a4987/41598_2017_7832_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17a2/5543151/7d9dc8bc6c5d/41598_2017_7832_Fig8_HTML.jpg

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