Sorbonne Université, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, 4 Place Jussieu, 75005 Paris, France.
AO Research Institute Davos, Davos Platz, Switzerland.
Carbohydr Polym. 2020 May 15;236:116042. doi: 10.1016/j.carbpol.2020.116042. Epub 2020 Feb 20.
A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties. At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels. At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed. These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration. Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability. These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils. Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
开发了一种具有可调节组成和凝胶条件的酶交联胶原/透明质酸衍生物（Col/HA-Tyr）水凝胶平台，以评估制备条件对其物理、化学和生物学特性的影响。在低 HA-Tyr 含量下，水凝胶表现出纤维状结构，与纯 Col 水凝胶相比，其力学性能较低。在高 HA-Tyr 和辣根过氧化物酶（HRP）含量下，除了带状 Col 纤维外，还形成了微纤维网络，并且观察到混合结构对机械性能的协同效应。这些水凝胶具有高度的抗酶降解性，同时保持高水合度。与 HA-Tyr 水凝胶不同的是，将人真皮成纤维细胞包封在 Col/HA-Tyr 水凝胶中可以保持较高的细胞活力。这些结果表明，需要高浓度的 HA-Tyr 和 HRP 来积极影响水凝胶的物理性质，同时保留胶原纤维。这些 Col/HA-Tyr 水凝胶有望通过仿生方法应用于新型组织工程。
