The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, PR China.
National Laboratory of Genomics for Biodiversity, CINVESTAV IPN, 36824, Irapuato, Guanajuato, Mexico.
Carbohydr Polym. 2020 May 15;236:116065. doi: 10.1016/j.carbpol.2020.116065. Epub 2020 Feb 24.
The present study aimed at investigating the structural features and antitumor properties of a novel heteropolysaccharide (CSP-W-2) obtained from the fruit of Chaenomeles Speciosa (Sweet) Nakai. CSP-W-2 demonstrate that they mainly contain glucose, galactose, arabinose, mannose, xylose in a ratio of 3.7: 3.2: 1.7: 0.9: 0.4, with the molecular weight of 8.7 kDa. Its backbone is predominantly composed of 1,4 linked β-D-Galp, 1,4 linked α-D-Glcp, 1,4 linked β-D-Glcp, and 1,4,6-β-D-Glcp, additionally some branches contained 1,5 linked α-L-Araf, 1,4 linked β-D-Glcp, 1,3 linked α-L-Araf, and T linked β-D-Manp according to the results of partial acid hydrolysis analysis, methylation analysis, IR and NMR spectra. The antitumor properties study results demonstrated that CSP-W-2 had an inhibitory effect on HepG2 growth by enhancing the nucleus shrinkage and apoptosis. These findings indicate that CSP-W-2 had antitumor potential in the treatment of human liver tumor.
本研究旨在探讨从木瓜（Chaenomeles speciosa（Sweet）Nakai）果实中获得的新型杂多糖（CSP-W-2）的结构特征和抗肿瘤特性。CSP-W-2 主要由葡萄糖、半乳糖、阿拉伯糖、甘露糖、木糖组成，摩尔比为 3.7:3.2:1.7:0.9:0.4，分子量为 8.7kDa。其主链主要由 1,4 连接的 β-D-Galp、1,4 连接的 α-D-Glcp、1,4 连接的 β-D-Glcp 和 1,4,6-β-D-Glcp 组成，根据部分酸水解分析、甲基化分析、IR 和 NMR 光谱的结果，还含有一些支链，包含 1,5 连接的 α-L-Araf、1,4 连接的 β-D-Glcp、1,3 连接的 α-L-Araf 和 T 连接的 β-D-Manp。抗肿瘤特性研究结果表明，CSP-W-2 通过增强核皱缩和细胞凋亡对 HepG2 生长具有抑制作用。这些发现表明 CSP-W-2 在治疗人类肝肿瘤方面具有抗肿瘤潜力。
