Biotechnology and Bioengineering Graduate Program, Izmir Institute of Technology, Gülbahçe Campus, 35430, Urla, Izmir, Turkey.
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, RG6 6AP, United Kingdom.
Carbohydr Polym. 2020 May 15;236:116076. doi: 10.1016/j.carbpol.2020.116076. Epub 2020 Feb 26.
Although most members of the genus Bifidobacterium are unable to utilize xylan as a carbon source, the growth of these species can be induced by this polysaccharide in the gut environment. This indicates a requirement for an association between Bifidobacterium species and some other members of gut microbiota. In this study, the role of cross-feeding between Bifidobacterium and Bacteroides species in the bifidogenic effect of xylan was investigated using in-vitro pure and co-culture fermentations. The pure culture studies showed that among the Bifidobacterium species tested, only Bifidobacterium animalis subsp. lactis was able to utilize xylooligosaccharides. The co-culture of this strain with Bacteroides species enabled it to grow in the presence of xylan. These results suggest that the ability of Bacteroides species to hydrolyze xylan could allow the proliferation of specific Bifidobacterium species in the gut through substrate cross-feeding.
虽然双歧杆菌属的大多数成员无法将木聚糖用作碳源，但这些物种的生长可以在肠道环境中被这种多糖诱导。这表明双歧杆菌属物种与肠道微生物群的某些其他成员之间存在共生关系的需求。在这项研究中，使用体外纯培养和共培养发酵研究了双歧杆菌和拟杆菌属物种之间的交叉喂养在木聚糖双歧杆菌效应中的作用。纯培养研究表明，在所测试的双歧杆菌属物种中，只有动物双歧杆菌亚种。乳双歧杆菌能够利用木二糖寡糖。该菌株与拟杆菌属的共培养使其能够在木聚糖存在的情况下生长。这些结果表明，拟杆菌属水解木聚糖的能力可以通过底物交叉喂养使特定的双歧杆菌属物种在肠道中增殖。
