中肠 RhoGAP 毒液蛋白抑制其宿主棉铃虫的细胞反应。
A RhoGAP venom protein from Microplitis mediator suppresses the cellular response of its host Helicoverpa armigera.
机构信息
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
出版信息
Dev Comp Immunol. 2020 Jul;108:103675. doi: 10.1016/j.dci.2020.103675. Epub 2020 Mar 12.
Female parasitoid wasps normally inject virulence factors together with eggs into their host to counter host immunity defenses. A newly identified RhoGAP protein in the venom of Microplitis mediator compromises the cellular immunity of its host, Helicoverpa armigera. RhoGAP1 proteins entered H. armigera hemocytes, and the host cellular cytoskeleton was disrupted. Depletion of MmGAP1 by injection of dsRNA or antibody increased the wasp egg encapsulation rate. An immunoprecipitation assay of overexpressed MmGAP1 protein in a Helicoverpa cell line showed that MmGAP1 interacts with many cellular cytoskeleton associated proteins as well as Rho GTPases. A yeast two-hybrid and a pull-down assay demonstrated that MmGAP1 interacts with H. armigera RhoA and Cdc42. These results show that the RhoGAP protein in M. mediator can destroy the H. armigera hemocyte cellular cytoskeleton, restrain host cellular immune defense, and increase the probability of successful parasitism.
雌性寄生蜂通常将毒力因子与卵一起注入宿主体内，以对抗宿主的免疫防御。在 Mediator 中的一种新鉴定的 RhoGAP 蛋白破坏了其宿主棉铃虫的细胞免疫。RhoGAP1 蛋白进入棉铃虫血细胞，破坏了宿主细胞的细胞骨架。通过注射 dsRNA 或抗体耗尽 MmGAP1 会增加蜂卵的包裹率。在一种棉铃虫细胞系中过表达 MmGAP1 蛋白的免疫沉淀试验表明，MmGAP1 与许多细胞骨架相关蛋白以及 Rho GTPases 相互作用。酵母双杂交和下拉测定表明，MmGAP1 与棉铃虫 RhoA 和 Cdc42 相互作用。这些结果表明 Mediator 中的 RhoGAP 蛋白可以破坏棉铃虫血细胞的细胞骨架，抑制宿主的细胞免疫防御，并增加成功寄生的可能性。
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