Aix Marseille Université, IRD, AP-HM, MEPHI, Marseille, France; IHU Méditerranée Infection, Marseille, France.
Aix Marseille Université, IRD, AP-HM, MEPHI, Marseille, France; IHU Méditerranée Infection, Marseille, France.
Microb Pathog. 2020 Jun;143:104140. doi: 10.1016/j.micpath.2020.104140. Epub 2020 Mar 12.
The study of ancient microorganisms represents one of the main ways to understand how microbes have evolved to date, especially those associated with humans or ecosystems of interest. However, these studies are always tedious because the viability of the microbes is difficult to maintain and the degradation of their DNA, can make their detection difficult. The explosion in the number of studies on ancient microorganisms in recent years is partly due to improved methods and their availability, ranging from microscopy to next generation sequencing techniques (NGS). In this article, we discuss these methods and their contribution to deciphering the ancient environmental microbial community, with particular emphasis on permafrost, ancient halite, amber and ancient rocks.
研究古代微生物是了解微生物至今如何进化的主要方法之一,尤其是那些与人类或感兴趣的生态系统相关的微生物。然而,这些研究总是很繁琐,因为微生物的生存能力难以维持,而且它们的 DNA 降解会使它们的检测变得困难。近年来,对古代微生物的研究数量的爆炸式增长部分归因于改进的方法及其可用性,从显微镜技术到下一代测序技术(NGS)。在本文中,我们讨论了这些方法及其对破译古代环境微生物群落的贡献,特别强调了永久冻土、古代岩盐、琥珀和古代岩石。