Institute for Nuclear Research, Atomki, Bem tér 18/c, 4026, Debrecen, Hungary; University of Debrecen, Doctoral School of Chemistry, Egyetem tér 1., 4032, Debrecen, Hungary.
University of Debrecen, Department of Agricultural Botany, Crop Physiolgy and Biotechnology, Institute of Crop Sciences, Egyetem tér 1., 4032, Debrecen, Hungary.
Appl Radiat Isot. 2020 Jun;160:109127. doi: 10.1016/j.apradiso.2020.109127. Epub 2020 Mar 10.
Manganese is one of the most important essential micronutrients for the plants. To monitor its uptake and transport by radioactive tracking is a powerful method due to the no carrier added Mn in 10 moldm concentration range. The generally used method is to measure the radioactivity of cut parts of plants by gamma-spectrometry. Only few studies reported about noninvasive measurement, using pairs of detectors connected in coincidence. We use a full ring MiniPET machine for this purpose to dynamically visualize the uptake and distribution of the radionuclide in 4D. The results are controlled with the conventional gamma spectroscopy after chopping the plants into six parts. The study of stress tolerance initiated by PEG 6000 in different hybrids of maize is also presented as possible application for the phenotyping of plants by PET camera.
锰是植物最重要的必需微量元素之一。由于 10 毫摩尔/升浓度范围内没有添加载体的 Mn，因此使用放射性跟踪监测其摄取和运输是一种强大的方法。通常使用的方法是通过伽马谱仪测量植物切割部分的放射性。只有少数研究报告了使用连接在符合探测器上的对探测器进行非侵入性测量。为此，我们使用全环 MiniPET 机器在 4D 中动态可视化放射性核素的摄取和分布。用常规的伽马光谱法对植物进行六部分切割后，对结果进行控制。还介绍了通过 PEG 6000 引发的不同玉米杂种的胁迫耐受性研究，作为通过 PET 相机对植物进行表型分析的可能应用。
