Higuchi Kyoko, Kurita Keisuke, Sakai Takuro, Suzui Nobuo, Sasaki Minori, Katori Maya, Wakabayashi Yuna, Majima Yuta, Saito Akihiro, Ohyama Takuji, Kawachi Naoki
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Tokyo 156-8502, Japan.
Materials Sciences Research Center, Japan Atomic Energy Agency (JAEA), Ibaraki 319-1195, Japan.
Plants (Basel). 2022 Mar 18;11(6):817. doi: 10.3390/plants11060817.
Iron (Fe) is an essential trace element in plants; however, the available Fe in soil solution does not always satisfy the demand of plants. Genetic diversity in the rate of Fe uptake by plants has not been broadly surveyed among plant species or genotypes, although plants have developed various Fe acquisition mechanisms. The "live-autoradiography" technique with radioactive Fe was adopted to directly evaluate the uptake rate of Fe by barley cultivars from a nutrient solution containing a very low concentration of Fe. The uptake rate of Fe measured by live autoradiography was consistent with the accumulation of Fe-containing proteins on the thylakoid membrane. The results revealed that the ability to acquire Fe from the low-Fe solution was not always the sole determinant of tolerance to Fe deficiency among barley genotypes. The live-autoradiography system visualizes the distribution of β-ray-emitting nuclides and has flexibility in the shape of the field of view. This technique will strongly support phenotyping with regard to the long-distance transport of nutrient elements in the plant body.
铁（Fe）是植物必需的微量元素；然而，土壤溶液中可利用的铁并不总能满足植物的需求。尽管植物已形成多种铁获取机制，但尚未在植物物种或基因型中广泛调查植物铁吸收速率的遗传多样性。采用含放射性铁的“活体放射自显影”技术直接评估大麦品种从低铁营养液中的铁吸收速率。通过活体放射自显影测量的铁吸收速率与类囊体膜上含铁蛋白的积累一致。结果表明，从低铁溶液中获取铁的能力并不总是大麦基因型中铁缺乏耐受性的唯一决定因素。活体放射自显影系统可直观显示发射β射线的核素分布，并且在视野形状方面具有灵活性。该技术将有力支持植物体内营养元素长距离运输的表型分析。
