Federal Institute of Education, Science and Technology of Ceará, IFCE, Campus Sobral, 62042-030, Sobral, CE, Brazil.
Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, 13081-970, SP, Brazil.
J Environ Manage. 2020 Jun 1;263:110387. doi: 10.1016/j.jenvman.2020.110387. Epub 2020 Mar 9.
The aim of this study was to evaluate the microbial structure and phylogenetic diversity under the influence of nutritional conditions and hydraulic retention time (HRT) in fluidized bed reactors (FBR), operated in short HRT (8 h - FBR8; 12 h - FBR12) for linear alkylbenzene sulfonate (LAS) removal from laundry wastewater. After each phase, biofilm samples from FBR8 and FBR12 were submitted to microbial sequencing by Mi-Seq Illumina®. Higher LAS removal rates were observed after 313 days, achieving 99 ± 3% in FBR12 (22.5 ± 5.9 mg LAS/L affluent) and 93 ± 12% in FBR8 (20.6 ± 4.4 mg LAS/L affluent). Different modifications involving genera of bacteria were observed throughout the reactors operation. The identified microorganisms were, mostly, related to LAS degradation and nitrogen conversion such as Dechloromonas, Flavobacterium, Pseudomonas, and Zoogloea.
本研究旨在评估营养条件和水力停留时间（HRT）对流化床反应器（FBR）中微生物结构和系统发育多样性的影响，FBR 分别在较短的 HRT（8 h - FBR8；12 h - FBR12）下运行，以去除洗衣废水中的直链烷基苯磺酸盐（LAS）。在每个阶段之后，FBR8 和 FBR12 的生物膜样品均通过 Mi-Seq Illumina®进行微生物测序。在 313 天后观察到更高的 LAS 去除率，在 FBR12 中达到 99±3%（22.5±5.9 mg LAS/L 进水），在 FBR8 中达到 93±12%（20.6±4.4 mg LAS/L 进水）。在整个反应器运行过程中观察到涉及细菌属的不同变化。鉴定出的微生物主要与 LAS 降解和氮转化有关，如 Dechloromonas、Flavobacterium、Pseudomonas 和 Zoogloea。
