Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, 511443, China.
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, 511443, China.
Biosens Bioelectron. 2020 May 15;156:112147. doi: 10.1016/j.bios.2020.112147. Epub 2020 Mar 10.
Quantifying the microRNA (miRNA) level and manipulating them in complex samples, such as serum, is of intense interest because miRNAs are important diagnostic markers. Here, we demonstrate an optical microfiber integrating of untrasensitive detection function and local photothermal therapy potential. A nanointerface consisting of GO supported CuS nanoplates presented the localized surface plasmon resonance (LSPR) tuned to be consistent with the operation wavelength of the microfiber transducer. It enhanced the surface energy density of evanescent field, on which the miRNA sensing and therapy occurred. With evanescent field enhancement by the plasmonic nanointerface, the sensor exhibits an ultrahigh sensitivity for detecting microRNA at concentrations ranging from 0.1 aM to 10 pM. It is also capable of differentiating one-base mismatches of miRNA at ultralow concentrations (as low as 10 aM) in serum. The photothermal effect of nanointerface simultaneously endows the sensor with the potential for localized photothermal therapy. This work presents a possible approach for the in-situ integration of diagnosis and treatment in early stage.
量化微 RNA（miRNA）水平并在复杂样本（如血清）中对其进行操作，这是非常重要的，因为 miRNA 是重要的诊断标志物。在这里，我们展示了一种光学微光纤，它集成了超灵敏检测功能和局部光热治疗潜力。由 GO 支撑的 CuS 纳米板组成的纳米界面呈现出与微光纤换能器工作波长一致的局域表面等离子体共振（LSPR）。它增强了消逝场的表面能密度，miRNA 的传感和治疗就在这个表面能密度上发生。通过等离子体纳米界面的消逝场增强，该传感器在检测浓度范围从 0.1 aM 到 10 pM 的 miRNA 时具有超高灵敏度。它还能够在超低浓度（低至 10 aM）的血清中区分 miRNA 的一个碱基错配。纳米界面的光热效应同时赋予传感器进行局部光热治疗的潜力。这项工作提出了一种在早期阶段实现诊断和治疗原位集成的可能方法。
