Fang Fang, Li Yanpeng, Yang Liuyang, Li Liangye, Yan Zhijun, Sun Qizhen
School of Optical and Electronic Information & National Engineer Laboratory for Next Generation Internal Access System & Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.
Nanomaterials (Basel). 2020 Dec 9;10(12):2461. doi: 10.3390/nano10122461.
The determination of hemoglobin (Hb) level is indispensable in the pathological study of many blood diseases. Graphene oxide (GO), with its excellent optical properties and great biocompatibility, has attracted significant attention and been widely utilized in biochemical detection. Here, we report an ultrasensitive Hb sensor based on a graphene oxide (GO)-coated microfiber. The GO was utilized as a linking layer deposited on the microfiber surface, which can provide an enhanced local evanescent light field and abundant bonding sites for Hb molecules. The optical microfiber with a compact structure and a strong evanescent light field served as the platform for biosensing. The surface morphology characterized by optical microscope, scanning electron microscope, and Raman spectroscopy offers detailed evidence for the success of GO deposition. The dynamic bonding between GO and target Hb molecules was monitored in real-time through an optical spectrum analyzer. An ultrahigh sensitivity of 6.02 nm/(mg/mL) with a detection limit of 0.17 μg/mL was achieved by tracking the resonant wavelength shift of spectra. It is important to highlight that the detection limit of GO-coated microfiber is 1-2 orders of magnitude lower than other reported fiber optic Hb sensors. Benefiting from high sensitivity, low cost, small size, and fast response, the proposed sensing microfiber coated with GO could be a competitive alternative in the diagnosis of blood diseases and a subject of further research in the medical field.
血红蛋白（Hb）水平的测定在许多血液疾病的病理学研究中不可或缺。氧化石墨烯（GO）具有优异的光学性质和良好的生物相容性，已引起广泛关注并被广泛应用于生化检测。在此，我们报道一种基于氧化石墨烯（GO）包覆微纤维的超灵敏Hb传感器。GO用作沉积在微纤维表面的连接层，可为Hb分子提供增强的局域倏逝光场和丰富的结合位点。具有紧凑结构和强倏逝光场的光学微纤维用作生物传感平台。通过光学显微镜、扫描电子显微镜和拉曼光谱表征的表面形态为GO沉积的成功提供了详细证据。通过光谱分析仪实时监测GO与目标Hb分子之间的动态结合。通过跟踪光谱的共振波长位移，实现了6.02 nm/（mg/mL）的超高灵敏度，检测限为0.17 μg/mL。需要强调的是，GO包覆微纤维的检测限比其他报道的光纤Hb传感器低1 - 2个数量级。得益于高灵敏度、低成本、小尺寸和快速响应，所提出的GO包覆传感微纤维在血液疾病诊断中可能是一种有竞争力的替代方案，也是医学领域进一步研究的课题。
