State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 210009, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 210009, PR China.
Biosens Bioelectron. 2020 May 15;156:112145. doi: 10.1016/j.bios.2020.112145. Epub 2020 Mar 16.
Currently, the large-scale and controllable fabrication of nanostructures on substrates remains a great challenge for further practical applications. In this work, a novel 3D aloe-like Au-ZnO nanocomposite was designed for in situ synthesis on an ITO substrate, achieving real-time detection of trace catechol (CC) in water. A seed-assisted hydrothermal approach was proposed to control the crystal distribution and growth direction to build a ZnO aloe-like architecture. To eliminate the natural weak conductivity of ZnO, Au nanoparticles were further deposited on all ZnO arrays to construct Au-ZnO micro/nanostructures. The synergetic effects derived from the aloe-like ZnO with a large specific area and Au nanoparticles with high conductivity resulted in both high electrocatalysis and fast electron transfer in enzymatic reactions. After laccase immobilization, the as-prepared biosensor exhibited specific recognition of catechol among other dihydroxybenzenes and phenol with an ultrahigh sensitivity of 131 μA mM, as well as an extremely wide linear range from 75 nM to 1100 μM and an ultralow detection limit of 25 nM. In addition, in the detection of real lake samples, this biosensor showed satisfactory anti-interference ability and provided reliable assay results.
目前，在基底上大规模且可控地制造纳米结构仍然是进一步实际应用的巨大挑战。在这项工作中，设计了一种新颖的 3D 芦荟状 Au-ZnO 纳米复合材料，用于在 ITO 基底上原位合成，实现了水中痕量儿茶酚（CC）的实时检测。提出了一种种子辅助水热法来控制晶体分布和生长方向，以构建 ZnO 芦荟状结构。为了消除 ZnO 固有的天然弱导电性，进一步在所有 ZnO 阵列上沉积了 Au 纳米颗粒，以构建 Au-ZnO 微/纳米结构。源于具有大比表面积的芦荟状 ZnO 和具有高导电性的 Au 纳米颗粒的协同效应导致了酶反应中的高电催化和快速电子转移。固定化漆酶后，所制备的生物传感器对儿茶酚表现出了特定的识别能力，在其他二羟基苯和苯酚中具有超高的灵敏度为 131 μA mM，以及从 75 nM 到 1100 μM 的极宽线性范围和超低检测限为 25 nM。此外，在真实湖泊样品的检测中，该生物传感器表现出令人满意的抗干扰能力并提供可靠的分析结果。
