Kumar Ritesh, Yadav Suresh Chandra, Kumar Sandeep, Dilbaghi Neeraj
1Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001 India.
2National Research Centre on Equines, Hisar, Haryana 125001 India.
J Parasit Dis. 2020 Mar;44(1):99-104. doi: 10.1007/s12639-019-01166-8. Epub 2019 Nov 8.
A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti- antibodies in equine sera and can be used for quick screening of serum samples under field conditions.
开发了一种使用蛋白A标记的金纳米颗粒（GNPs）的快速流通免疫分析法，用于定性检测马血清样本中的锥虫病。流通装置由固定在吸水垫上的抗原包被硝酸纤维素膜组成。基于GNPs的检测探针用作显色标记。该检测方法可用于检测感染马的混合血清样本在感染后14天的特异性抗体。该检测可在5分钟内完成。流通检测的结果与ELISA、斑点印迹法和侧向流动分析法等方法相当。所开发的检测方法在检测马血清中的抗体方面显示出高效率，可用于现场条件下血清样本的快速筛查。
