Bokharaei-Salim Farah, Esteghamati Abdoulreza, Khanaliha Khadijeh, Kalantari Saeed, Sayyahfar Shirin, Donyavi Tahereh, Garshasbi Saba, Asgari Qasem, Salemi Borna
1Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
2Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
J Parasit Dis. 2020 Mar;44(1):159-165. doi: 10.1007/s12639-019-01176-6. Epub 2019 Dec 11.
Cerebral toxoplasmosis is one of the neurological infections with high morbidity and mortality in patients with AIDS, so the accurate method for diagnosis of cerebral toxoplasmosis seems necessary. In this study, nested PCR assay using B1 gene was evaluated in diagnosis of toxoplasmosis in serum and peripheral blood mononuclear cell (PBMC) among HIV/AIDS patients. One hundred eight blood samples from HIV/AIDS patients, including four patients with cerebral toxoplasmosis and 104 HIV/AIDS patients without cerebral toxoplasmosis were evaluated for the antibodies using Enzyme Linked immunosorbent Assay. DNA of serum and PBMC of these patients were extracted and nested-PCR was carried out. Of 108 participants, 95 cases (88%) were positive for IgG antibodies and one patient was found positive for IgM antibody. In general, four patients, including three patients with cerebral toxoplasmosis, who were positive for IgG antibodies and one patient without cerebral toxoplasmosis who was positive for IgM antibody were found to be PCR positive. DNA of was detected in both serum and PBMC in two cerebral toxoplasmosis patients; however DNA was detected in only PBMC in other cerebral toxoplasmosis patient. All cases with cerebral toxoplasmosis were also diagnosed by clinical and radiological manifestations. The results of this study showed that the numbers of positive samples by PCR in PBMC were higher than serum specimens for diagnosis of toxoplasmosis. If molecular method and immunological assay are complemented with magnetic resonance imaging, the results can be useful for diagnosis of cerebral toxoplasmosis.
脑弓形虫病是艾滋病患者中发病率和死亡率较高的神经感染性疾病之一，因此准确诊断脑弓形虫病的方法显得很有必要。在本研究中，对采用B1基因的巢式PCR检测法在HIV/AIDS患者血清和外周血单个核细胞（PBMC）弓形虫病诊断中的应用进行了评估。采用酶联免疫吸附测定法对108份HIV/AIDS患者的血样进行抗体检测，其中包括4例脑弓形虫病患者和104例无脑弓形虫病的HIV/AIDS患者。提取这些患者血清和PBMC的DNA并进行巢式PCR。108名参与者中，95例（88%）IgG抗体呈阳性，1例患者IgM抗体呈阳性。总体而言，4例患者PCR呈阳性，其中包括3例IgG抗体阳性的脑弓形虫病患者和1例IgM抗体阳性的无脑弓形虫病患者。2例脑弓形虫病患者的血清和PBMC中均检测到DNA；然而，另1例脑弓形虫病患者仅在PBMC中检测到DNA。所有脑弓形虫病病例也均通过临床和影像学表现得以诊断。本研究结果表明，用于诊断弓形虫病时，PBMC中PCR阳性样本数高于血清标本。如果分子方法和免疫测定与磁共振成像相互补充，其结果将有助于脑弓形虫病的诊断。
