Takahashi Natsuki, Makino Ryosuke, Kita Kazumi
Department of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
Department of Agrobiological Science, Faculty of Agriculture, Ehime University, Matsuyama 790-8566, Japan.
J Poult Sci. 2020 Jan 25;57(1):63-66. doi: 10.2141/jpsa.0180150.
Eighty 14-d-old single-comb White Leghorn male chicks were divided into 16 groups with five birds each. Fructosyl-valine, which is a valine-glucose-Amadori product, was intravenously (2,250 nmol/kg body weight) or orally (300 mol/kg body weight) administered to chicks. Blood samples were collected 15, 30, 60, 120, 180, 360, 720 and 1440 min after administration. Plasma concentrations of fructosyl-valine were measured by using a liquid chromatography / mass spectrometry (LC/MS). The time course change in plasma fructosyl-valine concentration showed an exponential curve, as y=a+be. The half-life of plasma fructosyl-valine was calculated by the following equation: (log2)/. When fructosyl-valine was injected intravenously, the highest value for plasma fructosyl-valine concentration was observed 15 min after administration. When injected intravenously, the half-life of plasma fructosyl-valine was calculated to be 231 min. When fructosyl-valine was administered orally to chicks, the highest value for plasma fructosyl-valine concentration was observed 180 min after administration. When administered orally, the half-life of plasma fructosyl-valine was calculated to be 277 min. We conclude that the half-life of fructosyl-valine in plasma was approximately 4 h, which is longer than that of glycated tryptophan.
80只14日龄单冠白来航雄性雏鸡被分为16组，每组5只。将缬氨酸 - 葡萄糖 - 阿马多里产物果糖基缬氨酸以静脉注射（2250纳摩尔/千克体重）或口服（300微摩尔/千克体重）的方式给予雏鸡。给药后15、30、60、120、180、360、720和1440分钟采集血样。采用液相色谱/质谱联用（LC/MS）法测定血浆中果糖基缬氨酸的浓度。血浆果糖基缬氨酸浓度随时间的变化呈指数曲线，即y = a + be 。血浆果糖基缬氨酸的半衰期通过以下公式计算：(log2)/ 。静脉注射果糖基缬氨酸时，给药后15分钟观察到血浆果糖基缬氨酸浓度达到最高值。静脉注射时，计算得出血浆果糖基缬氨酸的半衰期为231分钟。当对雏鸡口服果糖基缬氨酸时，给药后180分钟观察到血浆果糖基缬氨酸浓度达到最高值。口服时，计算得出血浆果糖基缬氨酸的半衰期为277分钟。我们得出结论，血浆中果糖基缬氨酸的半衰期约为4小时，比糖化色氨酸的半衰期长。
