Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan.
Poult Sci. 2014 Feb;93(2):429-33. doi: 10.3382/ps.2013-03576.
Glycation starts from nonenzymatic amino-carbonyl reaction that binds carbonyl group of reducing sugars to the amino group of amino acids. Glycation leads to further complex reactions to form advanced glycation end products (AGE). Because AGE are implicated in the gradual development of diabetic complications, tissue accumulation of AGE has been widely examined in various tissues of rats. Avian species are known to have high body temperature and blood glucose concentration compared with mammals. Although these characteristics enabled chickens to be used as experimental models for diabetes mellitus, the information of AGE accumulation in various tissues of chickens has not been limited so far. In the present study, therefore, the radioactive AGE prepared by reacting (14)C-glucose and amino acids were intravenously administrated, and comparison of tissue accumulation of (14)C-labeled AGE was made between chickens and rats. At 30 min after administration, tissues (18-20) were collected, and the radioactivity incorporated into tissues was determined. High levels of radioactivity per gram of tissue in the liver and kidney were observed in both rats and chickens. In chickens but not rats, a large amount of (14)C-labeled AGE incorporated into 1 g of spleen was observed, and the specific accumulation of AGE in the avian spleen might have a particular role in immune response in avian species.
糖基化反应始于非酶促的氨基-羰基反应，该反应将还原糖的羰基基团与氨基酸的氨基基团结合。糖基化反应导致进一步的复杂反应，形成晚期糖基化终产物（AGE）。由于 AGE 与糖尿病并发症的逐渐发展有关，因此已在大鼠的各种组织中广泛检查了 AGE 的组织积累。与哺乳动物相比，禽类的体温和血糖浓度较高。尽管这些特征使鸡成为糖尿病实验模型，但迄今为止，有关鸡各种组织中 AGE 积累的信息还很有限。因此，在本研究中，通过将（14）C-葡萄糖和氨基酸反应制备放射性 AGE，并在鸡和大鼠之间比较（14）C 标记的 AGE 的组织积累。给药后 30 分钟，收集组织（18-20），并测定组织中的放射性。在大鼠和鸡中，肝脏和肾脏每克组织的放射性均很高。在鸡中，但不是在大鼠中，大量的（14）C 标记的 AGE 掺入 1g 脾脏中，并且禽类脾脏中 AGE 的特异性积累可能在禽类的免疫反应中具有特殊作用。
