Takahashi Natsuki, Kita Kazumi
Laboratory of Animal Nutrition, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
J Poult Sci. 2016 Apr 25;53(2):153-156. doi: 10.2141/jpsa.0150001.
Amadori products are non-enzymatically formed by binding carbonyl groups and amino groups. Glycated amino acids generated by reacting amino acid and glucose are also in a group of Amadori products of which the transport and metabolism have been investigated mainly in mammals but not in avians. In the present study, therefore, we examined whether dietary fructosyl-valine, which is one of the glycated amino acids, orally administrated to chickens can be incorporated into blood or not. Fructosyl-valine was orally administrated to the chicken and blood samples were collected at 0, 20, 40, 60, 120 and 180 min after administration. Plasma concentration of fructosyl-valine was measured by using LC/MS. The plasma concentration of fructosyl-valine was increased by passing time from 0 to 180 min after administration, and no change was observed in the control group. Conclusively, it was clarified that fructosyl-valine orally administrated to the chicken could be absorbed from gastrointestinal tract and incorporated into blood.
阿马多里产物是通过羰基与氨基结合非酶促形成的。由氨基酸与葡萄糖反应生成的糖化氨基酸也属于阿马多里产物，其转运和代谢主要在哺乳动物中进行了研究，而在禽类中尚未研究。因此，在本研究中，我们检测了口服给予鸡的糖化氨基酸之一——果糖基缬氨酸是否能进入血液。给鸡口服果糖基缬氨酸，并在给药后0、20、40、60、120和180分钟采集血样。使用液相色谱/质谱法测定血浆中果糖基缬氨酸的浓度。给药后从0到180分钟，血浆中果糖基缬氨酸的浓度随时间增加，而对照组未观察到变化。最终明确，口服给予鸡的果糖基缬氨酸可从胃肠道吸收并进入血液。
