Fan Rui, Tao Xiao-Yuan, Xia Zhi-Qiang, Sim Soonliang, Hu Li-Song, Wu Bao-Duo, Wang Qing-Huang, Hao Chao-Yun
Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Wanning, China.
Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2022 Jun 23;13:864927. doi: 10.3389/fpls.2022.864927. eCollection 2022.
is a destructive oomycete pathogen that causes devastating disease in black pepper, resulting in a significant decline in yield and economic losses. (black pepper) is documented as susceptible to , whereas its close relative is known to be resistant. However, the molecular mechanism underlying the resistance of remains obscure. In this study, we conducted a comparative transcriptome and metabolome analysis between and upon infection and found substantial differences in their gene expression profiles, with altered genes being significantly enriched in terms relating to plant-pathogen interaction, phytohormone signal transduction, and secondary metabolic pathways, including phenylpropanoid biosynthesis. Further metabolome analysis revealed the resistant to have a high background endogenous ABA reservoir and time-course-dependent accumulation of ABA and SA upon inoculation, while the susceptible had a high background endogenous IAA reservoir and time-course-dependent accumulation of JA-Ile, the active form of JA. Investigation of the phenylpropanoid biosynthesis metabolome further indicated the resistant to have more accumulation of lignin precursors than the susceptible , resulting in a higher accumulation after inoculation. This study provides an overall characterization of biologically important pathways underlying the resistance of , which theoretically explains the advantage of using this species as rootstock for the management of oomycete pathogen in black pepper production.
是一种具有破坏性的卵菌病原体，会导致黑胡椒毁灭性病害，造成产量大幅下降和经济损失。（黑胡椒）被记录为对 敏感，而其近缘种 已知具有抗性。然而， 抗性的分子机制仍不清楚。在本研究中，我们对 感染后的 和 进行了比较转录组和代谢组分析，发现它们的基因表达谱存在显著差异，差异基因在与植物 - 病原体相互作用、植物激素信号转导以及包括苯丙烷生物合成在内的次生代谢途径相关的术语中显著富集。进一步的代谢组分析表明，抗性 的内源脱落酸（ABA）储备较高，接种 后ABA和水杨酸（SA）呈时间进程依赖性积累，而敏感 的内源生长素（IAA）储备较高，接种后茉莉酸异亮氨酸（JA - Ile，JA的活性形式）呈时间进程依赖性积累。对苯丙烷生物合成代谢组的研究进一步表明，抗性 的木质素前体积累比敏感 的更多，接种后积累更高。本研究全面表征了 抗性的重要生物学途径，从理论上解释了将该物种用作黑胡椒生产中卵菌病原体管理砧木的优势。
