Salavati Saeed, Ahmadi Hedayati Manouchehr, Ahmadi Amjad, Fakhari Shohreh, Jalili Ali
Liver and Digestive Research Center, Reaserch Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Int J Prev Med. 2020 Feb 17;11:20. doi: 10.4103/ijpvm.IJPVM_536_18. eCollection 2020.
The correlation of infection with gastritis, peptic ulcer, and gastric cancer has been proven. The aim of this study was to determine the effects of and genotypes of on genes expression of interleukin (IL) -10 and tumor growth factor (TGF) β1 in gastric epithelial cells of patients with gastritis and infection.
In all, 45 gastric biopsy samples were collected from patients with gastritis and infection admitted to Tohid Hospital in Sanandaj city. Status of and genes of were directly determined from the biopsy samples using polymerase chain reaction (PCR) method. Expression of IL-10 and TGF-β1 genes in gastric epithelial cells of patients with gastritis and and cagA genotypes of infection was serveyed using real-time PCR method.
Overall, 25 samples had infection with and 20 with genotypes. This study showed that there is a positive correlation between genotypes of and increasing of IL-10 gene expression in gastric epithelial cells of patients with gastritis ( = 0.001).
Level of gene expression of IL-10 as an anti-inflammatory cytokine in gastric epithelial cells of patients with infection is connected to genotypes.
感染与胃炎、消化性溃疡及胃癌之间的相关性已得到证实。本研究旨在确定幽门螺杆菌的vacA和cagA基因型对胃炎及幽门螺杆菌感染患者胃上皮细胞中白细胞介素（IL）-10和肿瘤生长因子（TGF）β1基因表达的影响。
共收集了来自萨南达杰市托希德医院收治的胃炎及幽门螺杆菌感染患者的45份胃活检样本。采用聚合酶链反应（PCR）方法直接从活检样本中确定幽门螺杆菌的vacA和cagA基因状态。采用实时PCR方法检测胃炎患者胃上皮细胞中IL-10和TGF-β1基因的表达以及幽门螺杆菌感染的vacA和cagA基因型情况。
总体而言，25份样本感染了vacA基因型，20份感染了cagA基因型。本研究表明，幽门螺杆菌的cagA基因型与胃炎患者胃上皮细胞中IL-10基因表达增加之间存在正相关（P = 0.001）。
幽门螺杆菌感染患者胃上皮细胞中作为抗炎细胞因子的IL-10基因表达水平与cagA基因型有关。
