Zhu Yi, Lin Chunhui, Xu Huaming, Xia Zhaoxin, Yang Wensu, Tang Hao, Hu Xinyi, Jiang Tong, Liu Zhen, Shen Jilu
The First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.
Anhui Public Health Clinical Center, Hefei, People's Republic of China.
Infect Drug Resist. 2023 Jan 25;16:435-443. doi: 10.2147/IDR.S398098. eCollection 2023.
More than half of the world's people are infected or have been infected with . This infection is related to many diseases, with its pathogenicity related to virulence factors. Therefore, the rapid diagnosis of and genotyping of virulence genes play an extremely important role in the clinical treatment and control of transmission.
To this end, we developed a molecular detection method based on RPA- CRISPR-Cas12a technology for the specific genes 16S rDNA gene, cytotoxin associated gene A(), and vacuolating cytotoxin A () of
The results of which were displayed by lateral flow strips. Macroscopic observation takes only about 25 minutes and the sensitivity is 2ng/microliter.
The method is simple, convenient to operate and has low costs, and can therefore be applied widely to the detection and typing of in various environments such as primary hospitals, community clinics, outdoors, and large medical institutions.
世界上超过一半的人感染过或曾感染过[病原体名称未给出]。这种感染与多种疾病相关，其致病性与毒力因子有关。因此，[病原体名称未给出]的快速诊断和毒力基因分型在临床治疗和传播控制中起着极其重要的作用。
为此，我们开发了一种基于RPA-CRISPR-Cas12a技术的分子检测方法，用于检测[病原体名称未给出]的特定基因16S rDNA基因、细胞毒素相关基因A（[基因名称未给出]）和空泡毒素A（[基因名称未给出]）。
结果通过侧流试纸条显示。肉眼观察仅需约25分钟，灵敏度为2纳克/微升。
该方法简单、操作方便且成本低，因此可广泛应用于基层医院、社区诊所、户外及大型医疗机构等各种环境中[病原体名称未给出]的检测和分型。
