Pawlowski Przemyslaw, Poplawska Izabela, Mysliwiec Janusz, Dik Willem A, Eckstein Anja, Berchner-Pfannschmidt Utta, Milewski Robert, Lawicki Slawomir, Dzieciol-Anikiej Zofia, Rejdak Robert, Reszec Joanna
Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Bialystok, Poland.
Department of Paediatric Ophthalmology and Strabismus, Medical University of Bialystok, Bialystok, Poland.
Folia Histochem Cytobiol. 2020;58(1):37-45. doi: 10.5603/FHC.a2020.0003. Epub 2020 Mar 16.
Graves' orbitopathy (GO) is a complication in Graves' disease (GD) that causes disfigurement and sometimes blindness. The pathogenesis of GO remains unknown, while its symptoms demonstrate dependence between the thyroid gland and the orbit. The ongoing inflammatory process in retrobulbar tissue results in its remodeling characterized by increased volume of the orbital contents involving adipose tissue, with fibrosis and adipogenesis as predominant features. This study was aimed at the immunohistochemical verification of potential contribution and correlation between orbital expressions of IGF-1R, CD34, Foxp-3, PPAR-γ and CD4, CD68, TGF-β, FGF-β in severe and mild (long-lasting) GO.
Forty-one orbital tissue specimens - 22 patients with severe GO, 9 patients with mild GO and 10 patients undergoing blepharoplasty as a control group - were processed by routine immunohistochemistry.
Increased IGF-1R, CD34 and Foxp-3 expression was found in both severe and mild GO, yet a significant correlation between CD34 and CD4, CD68, TGF-β, FGF-β expressions was observed in long-lasting GO.
CD34 expression is proposed to be the marker of orbital tissue remodeling in the course of mild GO.
格雷夫斯眼眶病（GO）是格雷夫斯病（GD）的一种并发症，可导致容貌毁损，有时甚至失明。GO的发病机制尚不清楚，但其症状显示甲状腺与眼眶之间存在关联。球后组织中持续的炎症过程导致其重塑，其特征是眼眶内容物（包括脂肪组织）体积增加，以纤维化和脂肪生成为主要特征。本研究旨在通过免疫组织化学方法验证严重和轻度（长期）GO中IGF-1R、CD34、Foxp-3、PPAR-γ与CD4、CD68、TGF-β、FGF-β眼眶表达之间的潜在作用和相关性。
41份眼眶组织标本——22例严重GO患者、9例轻度GO患者以及10例行眼睑成形术的患者作为对照组——采用常规免疫组织化学方法进行处理。
在严重和轻度GO中均发现IGF-1R、CD34和Foxp-3表达增加，但在长期GO中观察到CD34与CD4、CD68、TGF-β、FGF-β表达之间存在显著相关性。
CD34表达被认为是轻度GO病程中眼眶组织重塑的标志物。
