Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China.
College of Fisheries, Henan Normal University, Xinxiang, China.
Zebrafish. 2020 Apr;17(2):91-103. doi: 10.1089/zeb.2019.1843. Epub 2020 Mar 16.
Spring viremia of carp virus (SVCV) causes the skin hemorrhagic disease in cyprinid species, but its molecular mechanism of skin immune response remains unclear at the protein level. In the present study, the differential proteomics of the zebrafish () skin in response to SVCV infection were examined by isobaric tags for relative and absolute quantitation and quantitative polymerase chain reaction (qPCR) assays. A total of 3999 proteins were identified, of which 320 and 181 proteins were differentially expressed at 24 and 96 h postinfection, respectively. The expression levels of 16 selected immune-related differentially expressed proteins (DEPs) were confirmed by qPCR analysis. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that DEPs were significantly associated with complement, inflammation, and antiviral response. The protein-protein interaction network of cytoskeleton-associated proteins, ATPase-related proteins, and parvalbumins from DEPs was shown to be involved in skin immune response. This is first report on the skin proteome profiling of zebrafish against SVCV infection, which will contribute to understand the molecular mechanism of local mucosal immunity in fish.
鲤春病毒血症病毒（SVCV）可引起鲤科鱼类的皮肤出血性疾病，但在蛋白质水平上，其皮肤免疫反应的分子机制尚不清楚。本研究采用相对和绝对定量同位素标记技术（iTRAQ）和定量聚合酶链反应（qPCR）技术，研究了 SVCV 感染对斑马鱼（）皮肤的差异蛋白质组学。共鉴定出 3999 种蛋白质，其中 24 和 96 h 感染后分别有 320 和 181 种蛋白质差异表达。通过 qPCR 分析验证了 16 种选定的免疫相关差异表达蛋白（DEPs）的表达水平。此外，GO 和京都基因与基因组百科全书富集分析表明，DEPs 与补体、炎症和抗病毒反应显著相关。从 DEPs 中细胞骨架相关蛋白、ATP 相关蛋白和副肌球蛋白的蛋白质-蛋白质相互作用网络表明其参与了皮肤免疫反应。这是首次报道针对 SVCV 感染的斑马鱼皮肤蛋白质组学，这将有助于了解鱼类局部黏膜免疫的分子机制。
