College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China; Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Fish Shellfish Immunol. 2017 Oct;69:35-45. doi: 10.1016/j.fsi.2017.07.055. Epub 2017 Jul 27.
Spring viremia of carp virus (SVCV) is the pathogen of spring viremia of carp (SVC) and often causes acute hemorrhagic symptoms in various kinds of cyprinids and induces serious environmental and economic losses. However, the molecular mechanisms of infection remain poorly understood, especially at the individual level. In this study, zebrafish was employed as the infection model to explore the pathogenesis of SVCV. 4 groups of zebrafish tissues were set and RNA sequencing (RNA-Seq) technology was employed to analyze the differentially expressed genes (DEGs) after SVCV-infection. A total of 360,971,498 clean reads were obtained from 12 samples, 382 DEGs in the brain and 926 DEGs in the spleen were identified. These DEGs were annotated into three ontologies after gene ontology (GO) enrichment analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these DEGs were primarily related to Influenza A pathway and Herpes simplex infection pathway in brain and Tuberculosis and Toxoplasmosis pathways in spleen, and all of these pathways may be involved in response to pathogen invasion. At the same time, 3' and 5' alternative splicing (AS) events were significantly up-regulated in the spleen. The transcriptome analysis results demonstrated changes and tissue-specific influences caused by SVCV in vivo, which provided us with more information to understand the complex relationships between SVCV and its host.
鲤春病毒血症病毒（SVCV）是引起鲤春病毒血症（SVC）的病原体，常导致各种鲤科鱼类出现急性出血症状，引发严重的环境和经济损失。然而，其感染的分子机制仍知之甚少，尤其是在个体水平上。本研究以斑马鱼为感染模型，探讨了 SVCV 的发病机制。共设置了 4 组斑马鱼组织，采用 RNA 测序（RNA-Seq）技术分析 SVCV 感染后差异表达基因（DEGs）。从 12 个样本中获得了 360,971,498 条清洁reads，在大脑中鉴定出 382 个 DEGs，在脾脏中鉴定出 926 个 DEGs。GO 富集分析后，这些 DEGs 被注释到三个本体中。KEGG 通路分析表明，这些 DEGs 主要与大脑中的流感 A 途径和单纯疱疹感染途径以及脾脏中的结核病和弓形体病途径相关，所有这些途径可能都参与了对病原体入侵的反应。同时，脾脏中的 3' 和 5' 可变剪接（AS）事件显著上调。转录组分析结果表明，SVCV 在体内引起了变化和组织特异性影响，为我们提供了更多信息来理解 SVCV 与其宿主之间复杂的关系。
