Unité de Recherche Résistance Induite et Bio-protection des Plantes-EA 4707, Université de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse-Bâtiment 18, BP 1039, 51687 Reims Cedex 2, France.
Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), L-4940 Hautcharage, Luxembourg.
Genes (Basel). 2020 Mar 12;11(3):300. doi: 10.3390/genes11030300.
() is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact. The cell wall is a crucial structure for the growth and development of fungi. Thus, understanding cell wall structure, composition and biosynthesis can help in designing new strategies for pest management. Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in . We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase. We have also analyzed the differential expression of these genes in spores and in the mycelium after cultivation on different media, as well as in the presence of strain PsJN, a plant growth-promoting bacterium (PGPB). The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB. Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in , thus serving as a basis for future molecular studies on this ascomycete.
（）是一种子囊菌，可引发网斑病，这是一种最严重的大麦病害，会造成重大的经济影响。细胞壁是真菌生长和发育的关键结构。因此，了解细胞壁的结构、组成和生物合成可以帮助设计新的害虫管理策略。尽管网斑病的严重性和经济影响很大，但这是首次对（）中与细胞壁相关的基因进行分析。我们已经鉴定出参与细胞壁多糖合成/重塑的关键基因，即几丁质、β-（1,3）-葡聚糖和混合连接葡聚糖合酶，以及内切/外切葡聚糖酶和丝裂原活化蛋白激酶。我们还分析了这些基因在不同培养基上培养的孢子和菌丝中的差异表达，以及在植物促生菌（PGPB）PsJN 菌株存在下的表达情况。靶向基因表达分析显示，PGPB 的应用可提高孢子和菌丝中的基因表达水平。除了分析关键的细胞壁相关基因外，本研究还鉴定了最适合归一化 qPCR 结果的内参基因，为今后对这种子囊菌进行分子研究奠定了基础。
