冷等离体子体和水飞蓟宾纳米乳剂激活人黑色素瘤细胞自噬。
Cold Atmospheric Plasma and Silymarin Nanoemulsion Activate Autophagy in Human Melanoma Cells.
机构信息
Plasma Bioscience Research Center, Applied Plasma Medicine Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, Korea.
Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, Delhi 110062, India.
出版信息
Int J Mol Sci. 2020 Mar 12;21(6):1939. doi: 10.3390/ijms21061939.
BACKGROUND
Autophagy is reported as a survival or death-promoting pathway that is highly debatable in different kinds of cancer. Here, we examined the co-effect of cold atmospheric plasma (CAP) and silymarin nanoemulsion (SN) treatment on G-361 human melanoma cells via autophagy induction.
METHODS
The temperature and pH of the media, along with the cell number, were evaluated. The intracellular glucose level and and downstream pathways were assessed. Autophagy-related genes, related transcriptional factors, and autophagy induction were estimated using confocal microscopy, flow cytometry, and ELISA.
RESULTS
CAP treatment increased the temperature and pH of the media, while its combination with SN resulted in a decrease in intracellular ATP with the downregulation of survival and transcriptional pathways. Co-treatment blocked downstream paths of survival pathways and reduced (2 times), (10 times), (5 times), (5 times), and (10 times). CAP and SN co-treated treatment modulates transcriptional factor expressions (, , , , and ) and specific genes (, , and ) related to autophagy induction.
CONCLUSION
CAP and SN together activate autophagy in G-361 cells by activating and pathways, expressing autophagy-related transcription factors and genes.
背景
自噬被认为是一种促进生存或促进死亡的途径，但在不同类型的癌症中存在很大争议。在这里，我们通过自噬诱导研究了大气压冷等离子体 (CAP) 和水飞蓟宾纳米乳 (SN) 联合处理对 G-361 人黑色素瘤细胞的共同作用。
方法
评估了培养基的温度和 pH 值以及细胞数量。评估了细胞内葡萄糖水平和下游途径。使用共聚焦显微镜、流式细胞术和 ELISA 评估自噬相关基因、相关转录因子和自噬诱导。
结果
CAP 处理会增加培养基的温度和 pH 值，而与 SN 联合处理会导致细胞内 ATP 减少，同时下调生存和转录途径。联合治疗阻断了生存途径的下游途径，并降低了 (2 倍)、 (10 倍)、 (5 倍)、 (5 倍) 和 (10 倍)。CAP 和 SN 共同处理调节与自噬诱导相关的转录因子表达（、、、和）和特定基因（、、和）。
结论
CAP 和 SN 通过激活和途径，表达自噬相关转录因子和基因，共同激活 G-361 细胞中的自噬。
Zotero 插件