Morita Aryan, Hamoh Thamir, Martinez Felipe P Perona, Chipaux Mayeul, Sigaeva Alina, Mignon Charles, Laan Kiran J van der, Hochstetter Axel, Schirhagl Romana
Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Nanomaterials (Basel). 2020 Mar 12;10(3):516. doi: 10.3390/nano10030516.
Fluorescent nanodiamonds are frequently used as biolabels. They have also recently been established for magnetic resonance and temperature sensing at the nanoscale level. To properly use them in cell biology, we first have to understand their intracellular fate. Here, we investigated, for the first time, what happens to diamond particles during and after cell division in yeast () cells. More concretely, our goal was to answer the question of whether nanodiamonds remain in the mother cells or end up in the daughter cells. Yeast cells are widely used as a model organism in aging and biotechnology research, and they are particularly interesting because their asymmetric cell division leads to morphologically different mother and daughter cells. Although yeast cells have a mechanism to prevent potentially harmful substances from entering the daughter cells, we found an increased number of diamond particles in daughter cells. Additionally, we found substantial excretion of particles, which has not been reported for mammalian cells. We also investigated what types of movement diamond particles undergo in the cells. Finally, we also compared bare nanodiamonds with lipid-coated diamonds, and there were no significant differences in respect to either movement or intracellular fate.
荧光纳米金刚石经常被用作生物标记物。它们最近还被用于纳米尺度的磁共振和温度传感。为了在细胞生物学中正确使用它们，我们首先必须了解它们在细胞内的命运。在这里，我们首次研究了酵母（）细胞在细胞分裂期间和之后金刚石颗粒会发生什么。更具体地说，我们的目标是回答纳米金刚石是留在母细胞中还是最终进入子细胞的问题。酵母细胞在衰老和生物技术研究中被广泛用作模式生物，它们特别有趣，因为它们的不对称细胞分裂会导致形态上不同的母细胞和子细胞。尽管酵母细胞有一种机制来防止潜在有害物质进入子细胞，但我们发现子细胞中的金刚石颗粒数量增加了。此外，我们发现了大量的颗粒排泄，这在哺乳动物细胞中尚未有报道。我们还研究了金刚石颗粒在细胞中经历的运动类型。最后，我们还比较了裸纳米金刚石和脂质包裹的金刚石，在运动或细胞内命运方面没有显著差异。
