Gwangju Bio/Energy Research Center, Korea Institute of Energy Research (KIER), Gwangju 61003, Republic of Korea.
Gwangju Bio/Energy Research Center, Korea Institute of Energy Research (KIER), Gwangju 61003, Republic of Korea; School of Chemical Engineering, Chonnam National University, Gwangju 61886, Republic of Korea.
Bioresour Technol. 2020 Jun;305:123155. doi: 10.1016/j.biortech.2020.123155. Epub 2020 Mar 6.
Due to climate change, recent research interests have increased towards CO utilization as a strategy to mitigate the atmospheric CO level. Herein, we aimed to explore formate dehydrogenases (FDHs) from chemoautotroph to discover an efficient and O-tolerant biocatalyst for catalyzing the CO reduction to a versatile formate. Through genome-mining and phylogenetic analysis, the FDH from Rhodobacter aestuarii (RaFDH) was newly discovered as a promising O-tolernat CO reductase and was successfully expressed in Escherichia coli. In this study, the optimum conditions and turnover rates of RaFDH were examined for CO reduction and formate oxidation. In particular, the RaFDH-driven CO reduction far surpassed the formate oxidation with a turnover rate of 48.3 and 15.6 min, respectively. The outstanding superiority of RaFDH towards CO reduction can be applicable for constructing a feasible electroenzymatic system that produce a versatile formate from CO as a cheap, abundant, and renewable resource.
由于气候变化，最近的研究兴趣增加了对 CO 利用的研究，将其作为减轻大气 CO 水平的一种策略。在此，我们旨在探索化能自养生物中的甲酸脱氢酶（FDH），以发现一种高效且耐 O 的生物催化剂，用于催化 CO 还原为多功能的甲酸。通过基因组挖掘和系统发育分析，新发现了来自盐沼红杆菌（Rhodobacter aestuarii）的 FDH（RaFDH），它是一种很有前途的耐 O CO 还原酶，并在大肠杆菌中成功表达。在本研究中，我们研究了 RaFDH 用于 CO 还原和甲酸氧化的最佳条件和周转率。特别是，RaFDH 驱动的 CO 还原远远超过了甲酸氧化，其周转率分别为 48.3 和 15.6 min。RaFDH 对 CO 还原的优异优势可适用于构建一种可行的电酶系统，从 CO 作为一种廉价、丰富且可再生的资源生产多功能甲酸。
