Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research (KIER), Gwangju 61003, Republic of Korea.
Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research (KIER), Gwangju 61003, Republic of Korea.
Bioresour Technol. 2022 Mar;348:126832. doi: 10.1016/j.biortech.2022.126832. Epub 2022 Feb 8.
With concerns over global warming and climate change, many efforts have been devoted to mitigate atmospheric CO level. As a CO utilization strategy, formate dehydrogenase (FDH) from Clostridium species were explored to discover O-tolerant and efficient FDHs that can catalyze CO to formate (i.e. CO reductase). With FDH from Clostridium ljungdahlii (ClFDH) that plays as a CO reductase previously reported as the reference, FDH from C.autoethanogenum (CaFDH), C. coskatii (CcFDH), and C. ragsdalei (CrFDH) were newly discovered via genome-mining. The FDHs were expressed in Escherichia coli and the recombinant FDHs successfully catalyzed CO reduction with a specific activity of 15 U g-CaFDH, 17 U g-CcFDH, and 8.7 U g-CrFDH. Interestingly, all FDHs newly discovered retain their catalytic activity under aerobic condition, although Clostridium species are strict anaerobe. The results discussed herein can contribute to biocatalytic CO utilization.
由于对全球变暖和气候变化的担忧，人们投入了大量精力来降低大气 CO 水平。作为 CO 利用策略的一部分，探索了来自梭菌属（Clostridium）的甲酸脱氢酶（FDH），以发现对 O2 耐受且高效的 FDH，其可将 CO 催化转化为甲酸（即 CO 还原酶）。我们以先前报道的产甲酸 CO 还原酶的 Clostridium ljungdahlii（ClFDH）为参考，通过基因组挖掘，新发现了来自 C.autoethanogenum（CaFDH）、C. coskatii（CcFDH）和 C. ragsdalei（CrFDH）的 FDH。这些 FDH 在大肠杆菌中表达，重组 FDH 成功地催化了 CO 还原，其比活性分别为 15 U g-CaFDH、17 U g-CcFDH 和 8.7 U g-CrFDH。有趣的是，虽然梭菌属是严格的厌氧菌，但所有新发现的 FDH 在有氧条件下仍保留其催化活性。本文的研究结果有助于生物催化 CO 的利用。
