雌性性皮肤将二氢睾酮转化为雄烷二醇葡糖苷酸。

Conversion of dihydrotestosterone to androstanediol glucuronide by female sexual skin.

作者信息

Pasupuleti V, Lobo R, Horton R

机构信息

Department of Medicine, University of Southern California, Los Angeles 90033.

出版信息

Steroids. 1988 Mar-Apr;51(3-4):269-82. doi: 10.1016/0039-128x(88)90018-9.

DOI:10.1016/0039-128x(88)90018-9

PMID:3217954

Abstract

Sexual skin biopsies from 13 normal women were obtained and minces/3-h studied after adding either [3H]dihydrotestosterone (DHT) or [3H]androstanediol (3 alpha diol) to RPMI-1640 medium in a Dubnoff apparatus. Unconjugated or conjugated androgens (after hydrolysis) were purified by three chromatography steps. Formation of 3 alpha diol and 3 alpha diol glucuronide (3 alpha diolG) was linear with time. The conversion of DHT to DHT17 beta G was only 4.4 +/- 0.5%/200 mg/3 h, while conversion to 3 alpha diol was 32 +/- 1.7%. The back conversion of 3 alpha diol to DHT was 30 +/- 3% and conversion to 3 alpha diolG was 4.5 +/- 1.25%. The product of the conversion separately measured of DHT to 3 alpha diol and 3 alpha diol to 3 alpha diolG was 1.5%, which is not very different than the overall conversion rate of DHT to 3 alpha diolG of 1.4%. This study indicates that the predominant path in this tissue is DHT in equilibrium 3 alpha diol----3 alpha diolG, rather than formation of DHT17 beta G and then 3 alpha reduction to 3 alpha diolG.

摘要

取自13名正常女性的性皮肤活检样本被获取，并在杜氏培养箱中向RPMI - 1640培养基中添加[3H]双氢睾酮（DHT）或[3H]雄烷二醇（3α二醇）后切碎并研究3小时。未结合或结合的雄激素（水解后）通过三步色谱法进行纯化。3α二醇和3α二醇葡糖醛酸苷（3α二醇G）的形成与时间呈线性关系。DHT转化为DHT17βG的转化率仅为4.4±0.5%/200mg/3小时，而转化为3α二醇的转化率为32±1.7%。3α二醇逆向转化为DHT的转化率为30±3%，转化为3α二醇G的转化率为4.5±1.25%。分别测量的DHT转化为3α二醇以及3α二醇转化为3α二醇G的产物转化率为1.5%，这与DHT转化为3α二醇G的总体转化率1.4%没有太大差异。这项研究表明，该组织中的主要途径是DHT与3α二醇——3α二醇G处于平衡状态，而不是先形成DHT17βG，然后3α还原为3α二醇G。