Oncology Data Analytics Program, Catalan Institute of Oncology (ICO), Barcelona, Spain.
Colorectal Cancer Group, ONCOBELL Program, Bellvitge Institute of Biomedical Research (IDIBELL), Barcelona, Spain.
Sci Data. 2020 Mar 16;7(1):92. doi: 10.1038/s41597-020-0427-5.
The gut microbiome has a fundamental role in human health and disease. However, studying the complex structure and function of the gut microbiome using next generation sequencing is challenging and prone to reproducibility problems. Here, we obtained cross-sectional colon biopsies and faecal samples from nine participants in our COLSCREEN study and sequenced them in high coverage using Illumina pair-end shotgun (for faecal samples) and IonTorrent 16S (for paired feces and colon biopsies) technologies. The metagenomes consisted of between 47 and 92 million reads per sample and the targeted sequencing covered more than 300 k reads per sample across seven hypervariable regions of the 16S gene. Our data is freely available and coupled with code for the presented metagenomic analysis using up-to-date bioinformatics algorithms. These results will add up to the informed insights into designing comprehensive microbiome analysis and also provide data for further testing for unambiguous gut microbiome analysis.
肠道微生物组在人类健康和疾病中起着至关重要的作用。然而，使用下一代测序技术研究肠道微生物组的复杂结构和功能具有挑战性，并且容易出现可重复性问题。在这里，我们从我们的 COLSCREEN 研究中的九名参与者中获得了横截面结肠活检和粪便样本，并使用 Illumina 配对末端 shotgun（用于粪便样本）和 IonTorrent 16S（用于配对的粪便和结肠活检）技术进行了高覆盖率测序。每个样本的宏基因组由 4700 万到 9200 万条reads 组成，靶向测序覆盖了 16S 基因的七个高变区，每个样本超过 300000 条reads。我们的数据是免费提供的，并与使用最新生物信息学算法呈现的宏基因组分析代码相结合。这些结果将有助于深入了解全面的微生物组分析设计，也为进一步进行明确的肠道微生物组分析提供了数据。
