Islet Cell Exocytosis, Lund University Diabetes Centre, Department of Clinical Sciences Malmö, Lund University, Malmö, Sweden.
Clinical Research Centre, Skåne University Hospital, Lund and Malmö, Sweden.
Methods Mol Biol. 2020;2128:25-54. doi: 10.1007/978-1-0716-0385-7_3.
The polygenic background of selectively bred diabetes models mimics the etiology of type 2 diabetes. So far, three different rodent models (Goto-Kakizaki rats, Nagoya-Shibata-Yasuda mice, and Oikawa-Nagao mice) have been established in the diabetes research field by continuous selective breeding for glucose tolerance from outbred rodent stocks. The origin of hyperglycemia in these rodents is mainly insulin secretion deficiency from the pancreatic β-cells and mild insulin resistance in insulin target organs. In this chapter, we summarize backgrounds and phenotypes of these rodent models to highlight their importance in diabetes research. Then, we introduce experimental methodologies to evaluate β-cell exocytosis as a putative common defect observed in these rodent models.
选择性繁育的糖尿病模型的多基因背景模拟了 2 型糖尿病的病因。到目前为止，已经通过从杂交种系中连续选择葡萄糖耐量，在糖尿病研究领域建立了三种不同的啮齿动物模型（Goto-Kakizaki 大鼠、Nagoya-Shibata-Yasuda 小鼠和 Oikawa-Nagao 小鼠）。这些啮齿动物的高血糖起源主要是来自胰腺β细胞的胰岛素分泌不足和胰岛素靶器官的轻度胰岛素抵抗。在本章中，我们总结了这些啮齿动物模型的背景和表型，以突出它们在糖尿病研究中的重要性。然后，我们介绍了评估β细胞胞吐作用的实验方法学，这是这些啮齿动物模型中观察到的一个潜在共同缺陷。
