罕见的移植部位：在啮齿动物和猴的眼前房内移植胰岛和胰岛类器官。

Uncommon Transplantation Sites: Transplantation of Islets and Islet Organoids in the Anterior Chamber of the Eye of Rodents and Monkeys.

机构信息

The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden.

Translational Pre-Clinical Model Platform, Singapore Eye Research Institute (SERI), Singapore, Singapore.

出版信息

Methods Mol Biol. 2023;2592:21-36. doi: 10.1007/978-1-0716-2807-2_2.

DOI:10.1007/978-1-0716-2807-2_2

PMID:36507983

Abstract

The anterior chamber of the eye is a highly vascularized and innervated location that is also particularly rich in oxygen and immune privileged. This uncommon transplantation site offers unique possibilities for the observation of the transplanted material as well as for local pharmacological intervention. Transplantation of islets and islet organoids to the anterior chamber of the eye of mice and monkeys facilitates a multitude of new approaches for research into islet physiology and pathophysiology and for the treatment of diabetes. We now present a short overview of the experimental possibilities and describe an updated protocol for transplantation of islets and islet organoids into mice and monkeys.

摘要

眼前房是一个高度血管化和神经支配的部位，也特别富含氧气和免疫特权。这个不常见的移植部位为观察移植材料以及局部药物干预提供了独特的可能性。将胰岛和胰岛类器官移植到小鼠和猴子的眼前房，为胰岛生理学和病理生理学的研究以及糖尿病的治疗提供了多种新方法。我们现在简要介绍一下实验的可能性，并描述一个将胰岛和胰岛类器官移植到小鼠和猴子体内的最新方案。