Department of Diabetes, School of Life Course Sciences, King's College London, London, UK.
Methods Mol Biol. 2020;2128:241-268. doi: 10.1007/978-1-0716-0385-7_17.
Islets of Langerhans are clusters of endocrine cells embedded within the exocrine pancreas. Islets constitute only approximately 1-2% of the total pancreas mass in all species, so methods have been developed to digest the pancreas and purify islets from the surrounding acinar cells. This chapter provides detailed protocols for isolation of mouse islets and their in vitro functional characterization in terms of assessments of islet viability, hormone content and secretion, second messenger generation and β-cell proliferation.
胰岛是嵌入外分泌胰腺中的内分泌细胞簇。胰岛仅占所有物种胰腺总质量的 1-2%左右，因此已经开发了一些方法来消化胰腺并从周围的腺泡细胞中纯化胰岛。本章提供了分离小鼠胰岛及其体外功能特征分析的详细方案，包括胰岛活力、激素含量和分泌、第二信使生成和β细胞增殖的评估。
