State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, 650500, P. R. China.
Chem Biodivers. 2020 May;17(5):e2000103. doi: 10.1002/cbdv.202000103. Epub 2020 Apr 22.
Three dimeric cassane diterpenoids, caesalpanins A-C, were isolated from the seeds of Caesalpinia sappan L., as well as three known compounds. Their structures were determined via analysis of 1D-, 2D-NMR, and HR-ESI-MS data. Caesalpanins A and B were the second and third compounds that presented a nitrogen-containing cassane diterpenoid dimer linked through one ether bond between C-19 and C-20'. Caesalpanin B exhibited moderate cytotoxic activity against MCF-7 cell lines with IC value of 29.98 μm. Caesalpanins A and B had weak inhibitory effects against LPS-induced nitric oxide (NO) production in RAW 264.7 macrophages at 50 μm with inhibitory rate of 36.01 % and 32.93 %, respectively.
从苏木（Caesalpinia sappan L.）种子中分离得到三种二聚型贝壳杉烷二萜类化合物，即 caesalpanins A-C，以及三种已知化合物。通过一维、二维 NMR 和高分辨电喷雾质谱（HR-ESI-MS）数据分析确定了它们的结构。Caesalpanins A 和 B 是第二个和第三个含有氮的贝壳杉烷二萜类二聚体，通过 C-19 和 C-20 之间的一个醚键连接。Caesalpanin B 对 MCF-7 细胞系表现出中等的细胞毒性，IC 值为 29.98 μm。Caesalpanins A 和 B 在 50 μm 时对 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）的产生具有较弱的抑制作用，抑制率分别为 36.01%和 32.93%。
