细颗粒物（PM2.5）通过促进角质形成细胞中胸腺基质淋巴细胞生成素（TSLP）的表达而成为皮炎的一个风险因素。
Fine Particulate Matter (PM2.5) is a Risk Factor for Dermatitis by Promoting the Expression of Thymic Stromal Lymphopoietin (TSLP) in Keratinocytes.
作者信息
Li Fei, Dong Yongpin, Ni Chunya, Kan Haidong, Yan Shuxian
机构信息
Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.
Fuyuan (Shanghai) Biotechnology Co. LTD, Shanghai, China.
出版信息
Indian J Dermatol. 2020 Mar-Apr;65(2):92-96. doi: 10.4103/ijd.IJD_520_18.
AIM
Common indoor pollutants, as fine particulate matter (PM2.5), can damage people's health and cause skin allergies. However, it remains unknown which common pollutants can lead to allergy, such as, in children atopic dermatitis, and what is the key molecule. This study aimed to investigate the thymic stromal lymphopoietin (TSLP) produced from keratinocytes after environmental pollutant stimulation.
METHODS
PAM212 cells were treated by several pollutants, including PM2.5, formaldehyde, m-xylene, and 1,2,4-trimethylbenzene, and tried to analyze their relationships. The mRNA expression level of TSLP was determined by qPCR. The protein level of TSLP was detected by ELISA analysis.
RESULTS
The mRNA expression of TSLP was significantly up-regulated when PAM212 cells were stimulated by PM2.5 at 25 μg/ml for 12 h. Meanwhile, the protein level of TSLP in culture supernatant was increased. However, TSLP protein production was not detected in culture supernatant treated with formaldehyde, m-xylene, and 1, 2, 4-trimethylbenzene.
CONCLUSION
PM2.5 promotes the expression of TSLP and may aggravate allergic response using this pathway.
目的
常见的室内污染物，如细颗粒物（PM2.5），会损害人们的健康并导致皮肤过敏。然而，尚不清楚哪些常见污染物会引发过敏，例如在儿童特应性皮炎中，以及关键分子是什么。本研究旨在调查环境污染物刺激后角质形成细胞产生的胸腺基质淋巴细胞生成素（TSLP）。
方法
用包括PM2.5、甲醛、间二甲苯和1,2,4-三甲基苯在内的几种污染物处理PAM212细胞，并试图分析它们之间的关系。通过qPCR测定TSLP的mRNA表达水平。通过ELISA分析检测TSLP的蛋白水平。
结果
当PAM212细胞用25μg/ml的PM2.5刺激12小时时，TSLP的mRNA表达显著上调。同时，培养上清液中TSLP的蛋白水平增加。然而，在用甲醛、间二甲苯和1,2,4-三甲基苯处理的培养上清液中未检测到TSLP蛋白产生。
结论
PM2.5促进TSLP的表达，并可能利用该途径加重过敏反应。
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