Li Qiao, Kang Zhihua, Jiang Shuo, Zhao Jinzhuo, Yan Shuxian, Xu Feng, Xu Jinhua
Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China.
Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China.
Int J Environ Res Public Health. 2017 Jan 12;14(1):72. doi: 10.3390/ijerph14010072.
The current study was conducted to observe the effects of fine particulate matter (PM) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM and skin was explored. HaCaT cells were cultured and then accessed in plate with PM. Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM. Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM. Ambient PM may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.
本研究旨在观察细颗粒物（PM）对人角质形成细胞系（HaCaT）细胞的影响。探讨了PM与皮肤之间潜在的关联机制。培养HaCaT细胞，然后用PM接种于培养板中。通过细胞计数试剂盒-8检测细胞活力。分析了丝聚合蛋白、兜甲蛋白、内披蛋白和重复蛋白的mRNA和蛋白表达。用酶联免疫吸附测定试剂盒检测HaCaT细胞上清液中粒细胞-巨噬细胞集落刺激因子、胸腺基质淋巴细胞生成素、肿瘤坏死因子-α、白细胞介素-1α和白细胞介素-8的水平。细胞活力随PM浓度增加而降低。与对照组相比，在PM处理组中，丝聚合蛋白、重复蛋白、内披蛋白和兜甲蛋白的蛋白表达呈现出不同的表达模式。经PM处理的细胞中，肿瘤坏死因子-α、胸腺基质淋巴细胞生成素、白细胞介素-1α和白细胞介素-8的水平显著升高。环境中的PM可能会增加患湿疹和其他皮肤病的风险。相关机制可能与皮肤屏障受损和炎症反应增强有关。
