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大鼠间脑投射至脑脊液接触核的单突触输入图谱

Monosynaptic Input Mapping of Diencephalic Projections to the Cerebrospinal Fluid-Contacting Nucleus in the Rat.

作者信息

Song Si-Yuan, Li Ying, Zhai Xiao-Meng, Li Yue-Hao, Bao Cheng-Yi, Shan Cheng-Jing, Hong Jia, Cao Jun-Li, Zhang Li-Cai

机构信息

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.

出版信息

Front Neuroanat. 2020 Feb 28;14:7. doi: 10.3389/fnana.2020.00007. eCollection 2020.

DOI:10.3389/fnana.2020.00007
PMID:32180709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7059736/
Abstract

: To investigate the projections the cerebrospinal fluid-contacting (CSF-contacting) nucleus receives from the diencephalon and to speculate on the functional significance of these connections. : The retrograde tracer cholera toxin B subunit (CB) was injected into the CSF-contacting nucleus in SD rats according to the experimental formula of the stereotaxic coordinates. Animals were perfused 7-10 days after the injection, and the diencephalon was sliced at 40 μm with a freezing microtome. CB-immunofluorescence was performed on all diencephalic sections. The features of CB-positive neuron distribution in the diencephalon were observed with a fluorescence microscope. : The retrograde labeled CB-positive neurons were found in the epithalamus, subthalamus, and hypothalamus. Three functional diencephalic areas including 43 sub-regions revealed projections to the CSF-contacting nucleus. The CB-positive neurons were distributed in different density ranges: sparse, moderate, and dense. : Based on the connectivity patterns of the CSF-contacting nucleus that receives anatomical inputs from the diencephalon, we preliminarily assume that the CSF-contacting nucleus participates in homeostasis regulation, visceral activity, stress, emotion, pain and addiction, and sleeping and arousal. The present study firstly illustrates the broad projections of the CSF-contacting nucleus from the diencephalon, which implies the complicated functions of the nucleus especially for the unique roles of coordination in neural and body fluids regulations.

摘要

目的

研究脑脊液接触核从间脑接受的投射，并推测这些联系的功能意义。

方法

按照立体定向坐标的实验公式，将逆行示踪剂霍乱毒素B亚单位（CB）注射到SD大鼠的脑脊液接触核中。注射后7-10天对动物进行灌注，用冷冻切片机将间脑切成40μm厚的切片。对所有间脑切片进行CB免疫荧光检测。用荧光显微镜观察间脑切片中CB阳性神经元分布的特征。

结果

在上丘脑、下丘脑和底丘脑发现了逆行标记的CB阳性神经元。包括43个亚区的三个功能性间脑区域显示出向脑脊液接触核的投射。CB阳性神经元分布在不同的密度范围内：稀疏、中等和密集。

结论

基于脑脊液接触核从间脑接受解剖学输入的连接模式，我们初步推测脑脊液接触核参与内环境稳态调节、内脏活动、应激、情绪、疼痛和成瘾以及睡眠和觉醒。本研究首次阐明了脑脊液接触核从间脑的广泛投射，这意味着该核的功能复杂，尤其是在神经和体液调节中的独特协调作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/53ab34c4a41e/fnana-14-00007-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/644f9a70732e/fnana-14-00007-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/b6b4740051c5/fnana-14-00007-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/84a38f97ecf9/fnana-14-00007-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/01414dec3ae8/fnana-14-00007-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/fce151d63819/fnana-14-00007-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/19283fa842df/fnana-14-00007-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/b3dd89231696/fnana-14-00007-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/550fd308c423/fnana-14-00007-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/319d1f8a2706/fnana-14-00007-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/53ab34c4a41e/fnana-14-00007-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/644f9a70732e/fnana-14-00007-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/b6b4740051c5/fnana-14-00007-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/84a38f97ecf9/fnana-14-00007-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/01414dec3ae8/fnana-14-00007-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/fce151d63819/fnana-14-00007-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/19283fa842df/fnana-14-00007-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/b3dd89231696/fnana-14-00007-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/550fd308c423/fnana-14-00007-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/319d1f8a2706/fnana-14-00007-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449e/7059736/53ab34c4a41e/fnana-14-00007-g0010.jpg

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