Zhang Li, Tang Junling, Sun Libo, Zheng Ting, Pu Xianzhi, Chen Yue, Yang Kai
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Department of Oral and Maxillofacial Surgery, Hospital of Stomatology Southwest Medical University, Luzhou, Sichuan, 646000, China.
Genes Dis. 2019 May 8;7(1):138-149. doi: 10.1016/j.gendis.2019.04.003. eCollection 2020 Mar.
Three-dimensional (3D) printed tissue engineered bone was used to repair the bone tissue defects in the oral and maxillofacial (OMF) region of experimental dogs.
Canine bone marrow stromal cells (BMSCs) were obtained from 9 male Beagle dogs and in vitro cultured for osteogenic differentiation. The OMF region was scanned for 3D printed surgical guide plate and mold by ProJet1200 high-precision printer using implant materials followed sintering at 1250 °C. The tissue engineered bones was co-cultured with BASCs for 2 or 8 d. The cell scaffold composite was placed in the defects and fixed in 9 dogs in 3 groups. Postoperative CT and/or micro-CT scans were performed to observe the osteogenesis and material degradation.
BMSCs were cultured with osteogenic differentiation in the second generation (P2). The nanoporous hydroxyapatite implant was made using the 3D printing mold with the white porous structure and the hard texture. BMSCs with osteogenic induction were densely covered with the surface of the material after co-culture and ECM was secreted to form calcium-like crystal nodules. The effect of the tissue engineered bone on the in vivo osteogenesis ability was no significant difference between 2 d and 8 d of the compositing time.
The tissue-engineered bone was constructed by 3D printing mold and high-temperature sintering to produce nanoporous hydroxyapatite scaffolds, which repair in situ bone defects in experimental dogs. The time of compositing for tissue engineered bone was reduced from 8 d to 2 d without the in vivo effect.
采用三维（3D）打印组织工程骨修复实验犬口腔颌面部（OMF）区域的骨组织缺损。
从9只雄性比格犬获取犬骨髓间充质干细胞（BMSCs）并进行体外成骨分化培养。使用植入材料通过ProJet1200高精度打印机对OMF区域进行扫描以制作3D打印手术导板和模具，随后在1250℃烧结。将组织工程骨与BASCs共培养2天或8天。将细胞支架复合材料置于缺损处并固定于9只犬，分为3组。术后进行CT和/或显微CT扫描以观察成骨情况和材料降解情况。
BMSCs在第二代（P2）时进行了成骨分化培养。使用3D打印模具制作出具有白色多孔结构和坚硬质地的纳米多孔羟基磷灰石植入物。成骨诱导后的BMSCs在共培养后密集覆盖于材料表面，并分泌细胞外基质形成类钙晶体结节。组织工程骨在体内成骨能力方面，复合材料培养2天和8天的效果无显著差异。
通过3D打印模具和高温烧结构建组织工程骨，制备出纳米多孔羟基磷灰石支架，可修复实验犬原位骨缺损。组织工程骨的复合材料培养时间从8天缩短至2天，且不影响体内效果。
