Center for Ecological Research, Northeast Forestry University, Heilongjiang Province, Harbin, 150040, China; Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization, Qiqihar, 161006, China; College of Life Sciences, Agriculture and Forestry, Qiqihar University, Heilongjiang Province, Qiqihar, 161006, China.
Heilongjiang Provincial Technology Innovation Center of Agromicrobial Preparation Industrialization, Qiqihar, 161006, China; College of Life Sciences, Agriculture and Forestry, Qiqihar University, Heilongjiang Province, Qiqihar, 161006, China.
Environ Pollut. 2020 Jul;262:114332. doi: 10.1016/j.envpol.2020.114332. Epub 2020 Mar 10.
Dibutyl phthalate (DBP) is widely used as plasticizer and has been detected in the environment, posing a threat to animal health. However, the effects of DBP on agricultural microbiomes are not known. In this study, DBP levels in black soil were evaluated, and the impact of DBP contamination on the uptake and metabolism of sugars in microbes was assessed by glucose absorption tests, metaproteomics, metabolomics, enzyme activity assays and computational simulation analysis. The results indicated that DBP contamination accelerated glucose consumption and upregulated the expression of porins and periplasmic monosaccharide ATP-binding cassette (ABC) transporter solute-binding proteins (SBPs). DBP and its metabolic intermediates (carboxymuconate and butanol) may form a stable complex with sugar transporters and enhance the rigidity and stability of these proteins. Sugar metabolism resulting in the generation of ATP and reducing agent (NADPH), as well as the expression of some key enzymes (dehydrogenases) were also upregulated by DBP treatment. Moreover, a diverse bacterial community appears to utilize sugar, suggesting that there are widespread effects of DBP contamination on soil microbial ecosystems. The results of this study provide a theoretical basis for investigating the toxicological effects of DBP on microbes in black soil.
邻苯二甲酸二丁酯（DBP）作为一种广泛使用的增塑剂，已在环境中被检出，对动物健康构成威胁。然而，DBP 对农业微生物组的影响尚不清楚。本研究评估了黑土中的 DBP 水平，并通过葡萄糖吸收试验、宏蛋白质组学、代谢组学、酶活性测定和计算模拟分析，评估了 DBP 污染对微生物摄取和代谢糖的影响。结果表明，DBP 污染加速了葡萄糖的消耗，并上调了孔蛋白和周质单糖 ATP 结合盒（ABC）转运体溶质结合蛋白（SBPs）的表达。DBP 及其代谢中间产物（羧基丁二酸和丁醇）可能与糖转运体形成稳定的复合物，增强这些蛋白质的刚性和稳定性。DBP 处理还上调了糖代谢产生的 ATP 和还原剂（NADPH），以及一些关键酶（脱氢酶）的表达。此外，多样化的细菌群落似乎利用糖，表明 DBP 污染对土壤微生物生态系统有广泛的影响。本研究结果为研究 DBP 对黑土中微生物的毒理学影响提供了理论依据。
