National Reference Laboratory for Arboviruses, Institut de Recherche Biomédicale des Armées, 13010 Marseille, France.
Unité des Virus Emergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13010 Marseille, France.
Viruses. 2020 Mar 9;12(3):296. doi: 10.3390/v12030296.
Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin.
2019 年 8 月，法国南部出现了三例 Zika 病毒本地感染病例。诊断依赖于血清学和转录介导的扩增。病毒分离和 ZIKV 基因组 RT-PCR 检测的尝试均为阴性。由于未确定首例病例，我们通过在 Pr 基因中进行半巢式 PCR 和测序来确定基因分型和地理来源。对 16 个基因型特异性单核苷酸多态性的分析确定了亚洲基因型，并提示该病毒源自东南亚。
