Al-Ajeeli Morouj N, Hubert Shawna M, Leyva-Jimenez Hector, Hashim Mohammed M, Abdaljaleel Raghad A, Alsadwi Akhil M, Athrey Giridhar, Bailey Christopher A
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA.
Calpis America, Inc. 455 Dividend Dr, Peachtree, GA 30269, USA.
Animals (Basel). 2020 Mar 9;10(3):453. doi: 10.3390/ani10030453.
The ingredients of poultry feeds are chosen based on the least-cost formulation to meet nutritional requirements. However, this approach can lead to the introduction of anti-nutritional ingredients in the feed. The objective of this study was to evaluate the impacts of two diets (with or without prebiotic) on homeostatic genes in the liver and spleen of laying hens. Hy-Line Brown layers were raised either on a soybean meal or cottonseed meal-based diets with and without an added prebiotic (yeast cell wall), totaling four experimental diets. A total of 120, 63-week old layers were housed individually in a wire cage system. We investigated differences in the expression of select homeostatic marker genes in the liver and spleen of hens from each treatment. We then used the ΔΔCT and generalized linear models to assess significance. Results show that the inclusion of prebiotic yeast cell-wall (YCW) increased the expression of the gene in the liver tissue for both the soybean meal (SBM) and cottonseed meal (CSM) diets. For splenic tissue, the combination of YCW with the CSM diet increased the POR gene over six log2 fold. Altogether, our results suggest altered homeostasis, which can have consequences for health and performance.
家禽饲料的成分是根据成本最低的配方选择的，以满足营养需求。然而，这种方法可能会导致饲料中引入抗营养成分。本研究的目的是评估两种日粮（添加或不添加益生元）对蛋鸡肝脏和脾脏中稳态基因的影响。海兰褐蛋鸡饲养在以豆粕或棉籽粕为基础的日粮中，添加或不添加益生元（酵母细胞壁），共四种实验日粮。总共120只63周龄的蛋鸡单独饲养在铁丝笼系统中。我们研究了每种处理的蛋鸡肝脏和脾脏中选定的稳态标记基因表达的差异。然后我们使用ΔΔCT和广义线性模型来评估显著性。结果表明，添加益生元酵母细胞壁（YCW）可增加豆粕（SBM）和棉籽粕（CSM）日粮肝脏组织中该基因的表达。对于脾脏组织，YCW与CSM日粮的组合使POR基因增加了超过6个log2倍。总之，我们的结果表明稳态发生了改变，这可能会对健康和性能产生影响。
