Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis.

We sought to examine the relationship between microtubule-associated proteins (MAPs) and the prognosis of urothelial carcinoma by assessing the microtubule bundle formation genes using a reappraisal transcriptome dataset of urothelial carcinoma (GSE31684). The result revealed that microtubule-associated protein 1b () is the most significant upregulated gene related to cancer progression. Real-time reverse-transcription polymerase chain reaction was used to measure transcription levels in urothelial carcinoma of the upper tract (UTUC) and the bladder (UBUC). Immunohistochemistry was conducted to detect protein expression in 340 UTUC and 295 UBUC cases. Correlations of expression with clinicopathological status, disease-specific survival, and metastasis-free survival were completed. To assess the oncogenic functions of , the RTCC1 and J82 cell lines were stably silenced against their endogenous expression. Study findings indicated that overexpression was associated with adverse clinical features and could independently predict unfavorable prognostic effects, indicating its theranostic value in urothelial carcinoma.