Bi Jingran, Tian Chuan, Zhang Gong-Liang, Hao Hongshun, Hou Hong-Man
School of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, Liaoning, China.
Liaoning Key Lab for Aquatic Processing Quality and Safety, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, Liaoning, China.
Foods. 2020 Mar 9;9(3):316. doi: 10.3390/foods9030316.
Gold nanoparticles (Au-NPs), with the dual sensor system of colorimetric and fluorescence responses, were developed for the determination of histamine as a spoilage monitor for distinguishing lifetime and freshness of aquatic products. Upon addition of histamine, the absorption coefficient orders of magnitude via the interaction of free electrons and photons were affected, and the characteristic absorption peak of Au-NPs was red-shifted from 520 nm to 664 nm. Meanwhile, the large amino groups in the networks of histamine-Au-NPs with high molecular orbital exhibited excellent fluorescence behavior at 415 nm. Au-NPs offered a range of 0.001-10.0 μM and 0.01-1.0 μM with a limit of detection of 0.87 nM and 2.04 nM by UV-vis and fluorescence spectrum assay, respectively. Moreover, Au-NPs could be used to semiquantitatively analyze histamine with the naked eye, since the significant colorimetric and fluorescence reaction of Au-NPs solution that coincided with different concentrations of histamine can be observed as the histamine concentration was 0.1-1.0 μM. Both of the dual-sensor systems of Au-NPs were successfully applied to the quantitative analysis of histamine in fresh salmon muscle, suggesting the simplicity and rapidity in the dual detection approaches of Au-NPs might be suitable for spoilage assay of aquatic food to ensure food safety.
开发了具有比色和荧光响应双传感器系统的金纳米颗粒（Au-NPs），用于测定组胺，作为区分水产品保质期和新鲜度的变质监测指标。加入组胺后，自由电子与光子相互作用导致吸收系数发生几个数量级的变化，Au-NPs的特征吸收峰从520 nm红移至664 nm。同时，组胺-Au-NPs网络中具有高分子轨道的大氨基在415 nm处表现出优异的荧光行为。通过紫外-可见光谱和荧光光谱分析，Au-NPs的检测范围分别为0.001-10.0 μM和0.01-1.0 μM，检测限分别为0.87 nM和2.04 nM。此外，由于当组胺浓度为0.1-1.0 μM时，可以观察到Au-NPs溶液与不同浓度组胺的显著比色和荧光反应，因此Au-NPs可用于肉眼半定量分析组胺。Au-NPs的双传感器系统均成功应用于新鲜三文鱼肌肉中组胺的定量分析，表明Au-NPs双检测方法的简单性和快速性可能适用于水产食品的变质检测，以确保食品安全。
