Lee Han, Liao Jiunn-Der, Sivashanmugan Kundan, Liu Bernard Haochih, Fu Wei-En, Chen Chih-Chien, Chen Guo Dung, Juang Yung-Der
Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan.
Medical Device Innovation Center, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan.
Nanomaterials (Basel). 2018 Jun 3;8(6):402. doi: 10.3390/nano8060402.
Trace detection of common pesticide residue is necessary to assure safety of fruit and vegetables, given that the potential health risk to consumers is attributed to the contamination of the sources. A simple, rapid and effective means of finding the residue is however required for household purposes. In recent years, the technique in association with surface-enhanced Raman scattering (SERS) has been well developed in particular for trace detection of target molecules. Herein, gold nanoparticles (Au NPs) were integrated with sol-gel spin-coated Zirconia nanofibers (ZrO₂ NFs) as a chemically stable substrate and used for SERS application. The morphologies of Au NPs/ZrO₂ NFs were adjusted by the precursor concentrations (_X, X = 0.05⁻0.5 M) and the effect of SERS on Au NPs/ZrO₂ NFs_X was evaluated by different Raman laser wavelengths using rhodamine 6G as the probe molecule at low concentrations. The target pesticides, phosmet (P1), carbaryl (C1), permethrin (P2) and cypermethrin (C2) were thereafter tested and analyzed. Au NPs/ZrO₂ NFs_0.3 exhibited an enhancement factor of 2.1 × 10⁷, which could detect P1, C1, P2 and C2 at the concentrations down to 10, 10, 10 and 10 M, respectively. High selectivity to the organophosphates was also found. As the pesticides were dip-coated on an apple and then measured on the diluted juice containing sliced apple peels, the characteristic peaks of each pesticide could be clearly identified. It is thus promising to use NPs/ZrO₂ NFs_0.3 as a novel SERS-active substrate for trace detection of pesticide residue upon, for example, fruits or vegetables.
鉴于消费者面临的潜在健康风险源于水果和蔬菜的源头污染，因此对常见农药残留进行痕量检测对于确保其安全至关重要。然而，家庭用途需要一种简单、快速且有效的方法来检测残留。近年来，特别是用于目标分子痕量检测的表面增强拉曼散射（SERS）相关技术得到了很好的发展。在此，金纳米颗粒（Au NPs）与溶胶 - 凝胶旋涂氧化锆纳米纤维（ZrO₂ NFs）集成作为化学稳定的基底，并用于SERS应用。通过前驱体浓度（_X，X = 0.05⁻0.5 M）调节Au NPs/ZrO₂ NFs的形貌，并以罗丹明6G作为低浓度探针分子，通过不同拉曼激光波长评估SERS对Au NPs/ZrO₂ NFs_X的影响。此后对目标农药稻丰散（P1）、西维因（C1）、氯菊酯（P2）和氯氰菊酯（C2）进行了测试和分析。Au NPs/ZrO₂ NFs_0.3表现出2.1×10⁷的增强因子，分别能够检测低至10、10、10和10 M浓度的P1、C1、P2和C2。还发现对有机磷酸酯具有高选择性。当将农药浸涂在苹果上，然后在含有苹果皮切片的稀释果汁中进行测量时，每种农药的特征峰都能清晰识别。因此，有望将NPs/ZrO₂ NFs_0.3用作新型SERS活性基底，用于例如水果或蔬菜上农药残留的痕量检测。
