• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

杂化树状聚合物在体外对正常和癌细胞神经元的代际效应和进入线粒体的作用。

Generation Dependent Effects and Entrance to Mitochondria of Hybrid Dendrimers on Normal and Cancer Neuronal Cells In Vitro.

机构信息

Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.

Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Campus Universitario, E-28871 Alcalá de Henares, Spain.

出版信息

Biomolecules. 2020 Mar 9;10(3):427. doi: 10.3390/biom10030427.

DOI:10.3390/biom10030427
PMID:32182909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7175207/
Abstract

Dendrimers as drug carriers can be utilized for drugs and siRNA delivery in central nervous system (CNS) disorders, including various types of cancers, such as neuroblastomas and gliomas. They have also been considered as drugs per se, for example as anti-Alzheimer's disease (AD), anti-cancer, anti-prion or anti-inflammatory agents. Since the influence of carbosilane-viologen-phosphorus dendrimers (SMT1 and SMT2) on the basic cellular processes of nerve cells had not been investigated, we examined the impact of two generations of these hybrid macromolecules on two murine cell lines-cancer cell line N2a (mouse neuroblastoma) and normal immortalized cell line mHippoE-18 (embryonic mouse hippocampal cell line). We examined alterations in cellular responses including the activity of mitochondrial dehydrogenases, the generation of reactive oxygen species (ROS), changes in mitochondrial membrane potential, and morphological modifications and fractions of apoptotic and dead cells. Our results show that both dendrimers at low concentrations affected the cancer cell line more than the normal one. Also, generation-dependent effects were found: the highest generation induced greater cytotoxic effects and morphological modifications. The most promising is that the changes in mitochondrial membrane potential and transmission electron microscopy (TEM) images indicate that dendrimer SMT1 can reach mitochondria. Thus, SMT1 and SMT2 seem to have potential as nanocarriers to mitochondria or anti-cancer drugs per se in CNS disorders.

摘要

树枝状聚合物作为药物载体可用于治疗中枢神经系统（CNS）疾病中的药物和 siRNA 传递，包括各种类型的癌症，如神经母细胞瘤和神经胶质瘤。它们也被认为是本身就是药物，例如抗阿尔茨海默病（AD）、抗癌、抗朊病毒或抗炎药物。由于碳硅烷-紫精-磷树枝状聚合物（SMT1 和 SMT2）对神经细胞基本细胞过程的影响尚未被研究，我们研究了两代这些杂化大分子对两种鼠细胞系-癌细胞系 N2a（鼠神经母细胞瘤）和正常永生化细胞系 mHippoE-18（胚胎鼠海马细胞系）的影响。我们检查了细胞反应的变化，包括线粒体脱氢酶的活性、活性氧（ROS）的产生、线粒体膜电位的变化以及形态修饰和凋亡和死亡细胞的分数。我们的结果表明，两种树枝状聚合物在低浓度下对癌细胞系的影响大于正常细胞系。此外，还发现了代际依赖性效应：最高代际诱导更大的细胞毒性作用和形态修饰。最有希望的是，线粒体膜电位的变化和透射电子显微镜（TEM）图像表明，树枝状聚合物 SMT1 可以到达线粒体。因此，SMT1 和 SMT2 似乎有可能作为纳米载体用于 CNS 疾病中的线粒体或抗癌药物本身。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/b4ae3c31a183/biomolecules-10-00427-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/37d2f1e46e5a/biomolecules-10-00427-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/c6a986ef6932/biomolecules-10-00427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/4738109a10d0/biomolecules-10-00427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/dcd2921d7b8c/biomolecules-10-00427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/27c33501831b/biomolecules-10-00427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/1e1b7159c986/biomolecules-10-00427-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/2e16fd9382e5/biomolecules-10-00427-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/7fa0d5192b86/biomolecules-10-00427-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/52055b2f95dd/biomolecules-10-00427-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/b4ae3c31a183/biomolecules-10-00427-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/37d2f1e46e5a/biomolecules-10-00427-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/c6a986ef6932/biomolecules-10-00427-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/4738109a10d0/biomolecules-10-00427-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/dcd2921d7b8c/biomolecules-10-00427-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/27c33501831b/biomolecules-10-00427-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/1e1b7159c986/biomolecules-10-00427-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/2e16fd9382e5/biomolecules-10-00427-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/7fa0d5192b86/biomolecules-10-00427-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/52055b2f95dd/biomolecules-10-00427-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5409/7175207/b4ae3c31a183/biomolecules-10-00427-g010.jpg

相似文献

1
Generation Dependent Effects and Entrance to Mitochondria of Hybrid Dendrimers on Normal and Cancer Neuronal Cells In Vitro.杂化树状聚合物在体外对正常和癌细胞神经元的代际效应和进入线粒体的作用。
Biomolecules. 2020 Mar 9;10(3):427. doi: 10.3390/biom10030427.
2
Viologen-phosphorus dendrimers exhibit minor toxicity against a murine neuroblastoma cell line.联吡啶磷树枝状聚合物对小鼠神经母细胞瘤细胞系的毒性较小。
Cell Mol Biol Lett. 2013 Sep;18(3):459-78. doi: 10.2478/s11658-013-0100-5. Epub 2013 Jul 26.
3
Mechanism of cationic phosphorus dendrimer toxicity against murine neural cell lines.阳离子磷树枝状大分子对小鼠神经细胞系的毒性机制。
Mol Pharm. 2013 Sep 3;10(9):3484-96. doi: 10.1021/mp4003255. Epub 2013 Jul 30.
4
Promising low-toxicity of viologen-phosphorus dendrimers against embryonic mouse hippocampal cells.具有前景的低毒的聚甲亚胺-磷树枝状大分子对胚胎鼠海马细胞。
Molecules. 2013 Sep 30;18(10):12222-40. doi: 10.3390/molecules181012222.
5
In vitro PAMAM, phosphorus and viologen-phosphorus dendrimers prevent rotenone-induced cell damage.体外 PAMAM、磷和紫精磷树枝状聚合物可预防鱼藤酮诱导的细胞损伤。
Int J Pharm. 2014 Oct 20;474(1-2):42-9. doi: 10.1016/j.ijpharm.2014.08.010. Epub 2014 Aug 7.
6
Ruthenium Dendrimers against Human Lymphoblastic Leukemia 1301 Cells.钌树状大分子抗人急性淋巴细胞白血病 1301 细胞。
Int J Mol Sci. 2020 Jun 9;21(11):4119. doi: 10.3390/ijms21114119.
7
Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage.碳硅烷树枝状聚合物抑制α-突触核蛋白纤维形成，并防止细胞遭受鱼藤酮诱导的损伤。
Int J Pharm. 2015 Apr 30;484(1-2):268-75. doi: 10.1016/j.ijpharm.2015.02.066. Epub 2015 Feb 28.
8
Ruthenium dendrimers against acute promyelocytic leukemia:  studies on HL-60 cells.钌树状大分子对抗急性早幼粒细胞白血病：对 HL-60 细胞的研究。
Future Med Chem. 2019 Jul;11(14):1741-1756. doi: 10.4155/fmc-2018-0274. Epub 2019 Jul 9.
9
Involvement of mitochondrial alteration and reactive oxygen species generation in Taiwan cobra cardiotoxin-induced apoptotic death of human neuroblastoma SK-N-SH cells.台湾眼镜蛇心脏毒素诱导人神经母细胞瘤SK-N-SH细胞凋亡死亡中线粒体改变和活性氧生成的作用。
Toxicon. 2008 Aug 1;52(2):361-8. doi: 10.1016/j.toxicon.2008.06.013. Epub 2008 Jun 24.
10
SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas.SapC-DOPS纳米囊泡通过激活神经母细胞瘤中的线粒体诱导Smac和Bax依赖性凋亡。
Mol Cancer. 2015 Apr 8;14:78. doi: 10.1186/s12943-015-0336-y.

引用本文的文献

1
Biocompatibility of Phosphorus Dendrimers and Their Antibacterial Properties as Potential Agents for Supporting Wound Healing.磷树枝状大分子的生物相容性及其作为促进伤口愈合潜在药物的抗菌特性
Mol Pharm. 2025 Feb 3;22(2):927-939. doi: 10.1021/acs.molpharmaceut.4c01156. Epub 2025 Jan 11.
2
The Proapoptotic Action of Pyrrolidinedione-Thiazolidinone Hybrids towards Human Breast Carcinoma Cells Does Not Depend on Their Genotype.吡咯烷二酮-噻唑烷酮杂化物对人乳腺癌细胞的促凋亡作用不依赖于其基因型。
Cancers (Basel). 2024 Aug 22;16(16):2924. doi: 10.3390/cancers16162924.
3
Multifunctional Nanocarriers for Alzheimer's Disease: Befriending the Barriers.

本文引用的文献

1
Dendrimers Show Promise for siRNA and microRNA Therapeutics.树枝状聚合物在小干扰RNA和微小RNA治疗方面展现出前景。
Pharmaceutics. 2018 Aug 8;10(3):126. doi: 10.3390/pharmaceutics10030126.
2
Binding of poly(amidoamine), carbosilane, phosphorus and hybrid dendrimers to thrombin-Constants and mechanisms.聚（酰胺胺）、碳硅烷、磷和杂化树枝状大分子与凝血酶的结合——常数及机制
Colloids Surf B Biointerfaces. 2017 Jul 1;155:11-16. doi: 10.1016/j.colsurfb.2017.03.053. Epub 2017 Mar 31.
3
Rational engineering of physicochemical properties of nanomaterials for biomedical applications with nanotoxicological perspectives.
多功能纳米载体治疗阿尔茨海默病：突破障碍。
Mol Neurobiol. 2024 May;61(5):3042-3089. doi: 10.1007/s12035-023-03730-z. Epub 2023 Nov 15.
4
A Glimpse into Dendrimers Integration in Cancer Imaging and Theranostics.树状高分子在癌症成像和治疗学中的应用一瞥。
Int J Mol Sci. 2023 Mar 12;24(6):5430. doi: 10.3390/ijms24065430.
5
Combination of Copper Metallodendrimers with Conventional Antitumor Drugs to Combat Cancer in In Vitro Models.铜金属树状大分子与传统抗肿瘤药物联合用于体外模型中的癌症治疗。
Int J Mol Sci. 2023 Feb 17;24(4):4076. doi: 10.3390/ijms24044076.
从纳米毒理学角度对用于生物医学应用的纳米材料的物理化学性质进行合理工程设计。
Nano Converg. 2016;3(1):1. doi: 10.1186/s40580-016-0064-z. Epub 2016 Feb 1.
4
Apoptin Gene Delivery by the Functionalized Polyamidoamine Dendrimer Derivatives Induces Cell Death of U87-MG Glioblastoma Cells.功能化聚酰胺-胺树枝状大分子衍生物介导凋亡素基因传递诱导U87-MG胶质母细胞瘤细胞死亡
J Pharm Sci. 2017 Jun;106(6):1618-1633. doi: 10.1016/j.xphs.2017.01.034. Epub 2017 Feb 7.
5
Dendrimer-protein interactions versus dendrimer-based nanomedicine.树枝状大分子与蛋白质的相互作用及基于树枝状大分子的纳米药物
Colloids Surf B Biointerfaces. 2017 Apr 1;152:414-422. doi: 10.1016/j.colsurfb.2017.01.041. Epub 2017 Jan 31.
6
Cancer's Achilles' Heel: Apoptosis and Necroptosis to the Rescue.癌症的致命弱点：凋亡与坏死性凋亡来拯救
Int J Mol Sci. 2016 Dec 23;18(1):23. doi: 10.3390/ijms18010023.
7
Synthesis of silica-PAMAM dendrimer nanoparticles as promising carriers in Neuro blastoma cells.二氧化硅-PAMAM树枝状聚合物纳米颗粒作为神经母细胞瘤细胞中有前景的载体的合成
Anal Biochem. 2017 Feb 15;519:1-7. doi: 10.1016/j.ab.2016.12.004. Epub 2016 Dec 8.
8
Progress and perspectives on targeting nanoparticles for brain drug delivery.用于脑药物递送的靶向纳米颗粒的研究进展与展望
Acta Pharm Sin B. 2016 Jul;6(4):268-86. doi: 10.1016/j.apsb.2016.05.013. Epub 2016 Jun 14.
9
Nanoparticle-mediated brain drug delivery: Overcoming blood-brain barrier to treat neurodegenerative diseases.纳米颗粒介导的脑内药物递送：克服血脑屏障治疗神经退行性疾病。
J Control Release. 2016 Aug 10;235:34-47. doi: 10.1016/j.jconrel.2016.05.044. Epub 2016 May 18.
10
Nanoparticles: Novel vehicles in treatment of Glioblastoma.纳米颗粒：治疗胶质母细胞瘤的新型载体。
Biomed Pharmacother. 2016 Feb;77:98-107. doi: 10.1016/j.biopha.2015.12.014. Epub 2015 Dec 29.