Roscoe Lucas E, Forbes Glen, Lamb Rosanna, Silk Peter J
Canadian Forest Service - Atlantic Forestry Centre, Natural Resources Canada, 1350 Regent Street, Fredericton, NB E3B 5P7, Canada.
Insects. 2020 Mar 14;11(3):184. doi: 10.3390/insects11030184.
(Clemens) (Lepidoptera: Tortricidae) is a defoliating pest in Canada and the northeastern United States. Given its important ecological and economic effects in affected regions, several direct management techniques have been developed, including the application of the insect growth regulator tebufenozide (Mimic™, RH-5992) to feeding larval stages. While the effectiveness of tebufenozide, in this capacity, is understood, management programs of other lepidopteran pests have demonstrated the effectiveness of tebufenozide application when utilized against other life stages. Here, we investigated the toxicity of topically-applied tebufenozide to pupae to determine if such a strategy could be feasible. We observed significant dose-dependent decreases in the likelihood of adult emergence, increases in the likelihood of pupal death or adult deformity at eclosion, and significant decreases in mean adult longevity. Estimated LD 50 (lethal dose) values for adult male and female treated as pupae ≤ 4 days after pupation were approximately 1-3 and 2-3.5% ACI (active commercial ingredient) respectively. Estimated L-SD (lethal-sublethal) 50 doses for adult male and female treated as pupae ≤4 days after pupation were <1, and <2% ACI, respectively. Mating success was also significantly lower in mating pairs containing adults treated as pupae. Although, the amounts required to cause appreciable pupal mortality were much higher than those currently applied operationally in the system, our study illustrates the potential of tebufenozide to utilized against additional developmental stages in other lepidopteran pests.
克莱门斯卷蛾（鳞翅目：卷蛾科）是加拿大和美国东北部的一种食叶害虫。鉴于其在受影响地区具有重要的生态和经济影响，已开发出几种直接管理技术，包括在幼虫取食阶段施用昆虫生长调节剂虫酰肼（Mimic™，RH - 5992）。虽然虫酰肼在此方面的有效性是已知的，但其他鳞翅目害虫的管理计划已证明，在针对其他生命阶段使用时，施用虫酰肼是有效的。在此，我们研究了局部施用虫酰肼对蛹的毒性，以确定这种策略是否可行。我们观察到成虫羽化可能性出现显著的剂量依赖性下降、羽化时蛹死亡或成虫畸形可能性增加，以及成虫平均寿命显著缩短。化蛹后≤4天作为蛹处理的成年雄性和雌性的估计半数致死剂量（LD50）值分别约为1 - 3%和2 - 3.5%的活性商业成分（ACI）。化蛹后≤4天作为蛹处理的成年雄性和雌性的估计致死 - 亚致死半数剂量（L - SD50）分别<1%和<2%的ACI。在包含作为蛹处理的成虫的交配对中，交配成功率也显著较低。虽然，导致明显蛹死亡率所需的量远高于目前该系统实际施用的量，但我们的研究表明虫酰肼在针对其他鳞翅目害虫的额外发育阶段方面具有潜力。
