Bratek-Skicki Anna
Institute of Condensed Matter and Nanosciences, Université catholique de Louvain, Place Louis Pasteur 1, bte L4.01.10, B-1348 Louvain-la-Neuve, Belgium.
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL30239 Krakow, Poland.
Polymers (Basel). 2020 Mar 15;12(3):660. doi: 10.3390/polym12030660.
Protein adsorption on solid surfaces provides either beneficial or adverse outcomes, depending on the application. Therefore, the desire to predict, control, and regulate protein adsorption on different surfaces is a major concern in the field of biomaterials. The most widely used surface modification approach to prevent or limit protein adsorption is based on the use of poly (ethylene oxide) (PEO). On the other hand, the amount of protein adsorbed on poly(2-(dimethylamine)ethyl methacrylate) (PDMAEMA) coatings can be regulated by the pH and ionic strength of the medium. In this work, ultra-thin PEO/PDMAEMA coatings were designed from solutions with different ratios of PEO to PDMAEMA, and different molar masses of PEO, to reversibly adsorb and desorb human serum albumin (HSA), human fibrinogen (Fb), lysozyme (Lys), and avidine (Av), four very different proteins in terms of size, shape, and isoelectric points. X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance (QCM), and atomic force microscopy (AFM) were used to characterize the mixed polymer coatings, revealing the presence of both polymers in the layers, in variable proportions according to the chosen parameters. Protein adsorption at pH 7.4 and salt concentrations of 10 M was monitored by QCM. Lys and Av did not adsorb on the homo-coatings and the mixed coatings. The amount of HSA and Fb adsorbed decreased with increasing the PEO ratio or its molar mass in a grafting solution. It was demonstrated that HSA and Fb, which were adsorbed at pH 7.4 and at an ionic strength of 10 M, can be fully desorbed by rinsing with a sodium chloride solution at pH 9.0 and ionic strength 0.15 M from the mixed PEO5/PDMAEMA coatings with PEO/PDMAEMA mass ratios of 70/30, and 50/50, respectively. The results demonstrate that mixed PEO/PDMAEMA coatings allow protein adsorption to be finely tuned on solid surfaces.
蛋白质在固体表面的吸附会产生有益或有害的结果，这取决于具体应用。因此，预测、控制和调节蛋白质在不同表面的吸附是生物材料领域的一个主要关注点。最广泛使用的防止或限制蛋白质吸附的表面改性方法是基于使用聚环氧乙烷（PEO）。另一方面，吸附在聚（甲基丙烯酸2-（二甲胺）乙酯）（PDMAEMA）涂层上的蛋白质数量可以通过介质的pH值和离子强度来调节。在这项工作中，从具有不同PEO与PDMAEMA比例以及不同摩尔质量的PEO的溶液中设计出超薄PEO/PDMAEMA涂层，以可逆地吸附和解吸人血清白蛋白（HSA）、人纤维蛋白原（Fb）、溶菌酶（Lys）和抗生物素蛋白（Av），这四种蛋白质在大小、形状和等电点方面差异很大。使用X射线光电子能谱（XPS）、石英晶体微天平（QCM）和原子力显微镜（AFM）来表征混合聚合物涂层，揭示了两层中两种聚合物的存在，其比例根据所选参数而变化。通过QCM监测在pH 7.4和盐浓度为10 M时的蛋白质吸附情况。Lys和Av没有吸附在均聚物涂层和混合涂层上。在接枝溶液中，随着PEO比例或其摩尔质量的增加，HSA和Fb的吸附量减少。结果表明，在pH 7.4和离子强度为10 M时吸附的HSA和Fb，可以通过用pH 9.0和离子强度0.15 M的氯化钠溶液冲洗，分别从PEO/PDMAEMA质量比为70/30和50/50的混合PEO5/PDMAEMA涂层上完全解吸。结果表明，混合PEO/PDMAEMA涂层能够在固体表面精细调节蛋白质吸附。
