Institute of Condensed Matter and Nanosciences, Université catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 Louvain-la-Neuve, Belgium.
Nanoscale. 2017 Nov 16;9(44):17186-17192. doi: 10.1039/c7nr04345g.
A standard method of protein immobilization is proposed, based on the use of protein-polyelectrolyte complexes (PPCs) as building blocks for layer-by-layer assembly. Thicker multilayers, with a higher polyelectrolyte fraction, are obtained with PPCs compared to single protein molecules. Biological activity is not only maintained, but specific activity is also higher, as demonstrated for lysozyme-poly(styrene sulfonate) complexes. This is attributed to the more hydrated state of the assemblies. This new method of protein immobilization opens up perspectives for biotechnology and biomedical applications.
提出了一种基于蛋白质-聚电解质复合物（PPC）作为逐层组装构建块的蛋白质固定化标准方法。与单个蛋白质分子相比，PPC 可获得更厚的多层和更高的聚电解质分数。不仅保持了生物活性，而且还提高了比活性，这在溶菌酶-聚苯乙烯磺酸盐复合物中得到了证明。这归因于组装体的更水合状态。这种新的蛋白质固定化方法为生物技术和生物医学应用开辟了前景。
