用单克隆抗体对小肠结肠炎耶尔森菌O:9和O:3脂多糖抗原进行免疫学特性分析。
Immunological characterization of Yersinia enterocolitica O:9 and O:3 LPS antigens by monoclonal antibodies.
作者信息
Weninger J, Wartenberg K, Röllinghoff M
机构信息
Institut für Klinische Mikrobiologie, Universität Erlangen-Nürnberg.
出版信息
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):298-313. doi: 10.1016/s0176-6724(88)80173-1.
LPS-specific monoclonal antibodies induced against Y. enterocolitica serotype O:9 bacteria and against Y. enterocolitica O:3 were used in a comparative study to characterize the O:9 and O:3 LPS. Yersinia bacteria grown at 22 degrees C and 37 degrees C and LPS preparations thereof were tested. SDS-PAGE, Western blot analysis and adsorption procedures revealed that LPS of Y. enterocolitica O:9 differed from that of Y. enterocolitica O:3 in: (i) its repeating LPS O-side-chain sugar, (ii) its shorter length of the LPS O-side-chain and (iii) its failure to show temperature-dependent variation in the length of O-side-chains.
针对小肠结肠炎耶尔森氏菌O:9血清型细菌和小肠结肠炎耶尔森氏菌O:3诱导产生的脂多糖特异性单克隆抗体，用于一项比较研究，以表征O:9和O:3脂多糖。测试了在22摄氏度和37摄氏度下生长的耶尔森氏菌及其脂多糖制剂。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、蛋白质免疫印迹分析和吸附程序显示，小肠结肠炎耶尔森氏菌O:9的脂多糖在以下方面与小肠结肠炎耶尔森氏菌O:3的脂多糖不同：(i) 其重复的脂多糖O侧链糖，(ii) 其脂多糖O侧链较短的长度，以及(iii) 其O侧链长度未显示出温度依赖性变化。
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