Bundle D R, Gidney M A, Perry M B, Duncan J R, Cherwonogrodzky J W
Infect Immun. 1984 Nov;46(2):389-93. doi: 10.1128/iai.46.2.389-393.1984.
Murine monoclonal antibodies that bind the O-antigens of Yersinia enterocolitica serotype O:9 and Brucella abortus 1119-3 were generated after immunization of BALB/c mice with killed, whole cells. Highly purified lipopolysaccharide preparations from each organism were used to screen for antigen-specific antibodies. Immunization with B. abortus cells induced 56 antigen-specific hybrids, and 10 of the highest antibody-producing clones were selected for further study. Seven of these clones secreted immunoglobulin G, and three secreted immunoglobulin M antibodies. Immunization with Y. enterocolitica cells resulted after fusion in 76 antigen-specific hybrid cell lines; from these, seven immunoglobulin G-secreting clones were selected for study. The serological cross-reactivity of the B. abortus and Y. enterocolitica O-antigens was established by enzyme-linked immunosorbent assay, immunoprecipitation, and agglutination tests with the monoclonal antibodies induced by each bacterium. This serological cross-reactivity is consistent with the structural identity of the two O-antigens established by chemical analysis.
用灭活的全细胞免疫BALB/c小鼠后，产生了与小肠结肠炎耶尔森菌O:9血清型和流产布鲁氏菌1119-3的O抗原结合的鼠单克隆抗体。使用来自每种生物体的高度纯化的脂多糖制剂筛选抗原特异性抗体。用流产布鲁氏菌细胞免疫诱导出56个抗原特异性杂交瘤，选择10个产生抗体能力最强的克隆进行进一步研究。其中7个克隆分泌免疫球蛋白G，3个分泌免疫球蛋白M抗体。用小肠结肠炎耶尔森菌细胞免疫后，融合产生了76个抗原特异性杂交细胞系；从中选择7个分泌免疫球蛋白G的克隆进行研究。通过酶联免疫吸附测定、免疫沉淀和用每种细菌诱导产生的单克隆抗体进行的凝集试验，确定了流产布鲁氏菌和小肠结肠炎耶尔森菌O抗原的血清学交叉反应性。这种血清学交叉反应性与化学分析确定的两种O抗原的结构一致性相符。
